Publication: Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II<sup>®</sup> CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia
Issued Date
2019-07-01
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ISSN
24055808
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2-s2.0-85064628676
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemistry and Biophysics Reports. Vol.18, (2019)
Suggested Citation
Uday Younis Hussein Abdullah, Hishamshah M. Ibrahim, Haitham Muhammed Jassim, Mohamad Zaki Salleh, Teh Lay Kek, Mohd Nur Fakhruzzaman bin Noorizhab, Bin Alwi Zilfalil, Prapin Wilairat, Suthat Fucharoen Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II<sup>®</sup> CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia. Biochemistry and Biophysics Reports. Vol.18, (2019). doi:10.1016/j.bbrep.2019.100635 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/50144
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Title
Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II<sup>®</sup> CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia
Abstract
© 2019 This is the first report of QQQ-mass spectrometric identification and quantification of the Hb subunits, alpha, beta, delta and gamma globin peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the Bio-Rad cation-exchange chromatography of haemoglobin. The objectives were to assess the relationship of the quantity of the free alpha, beta, delta and gamma globin chains with the phenotypic diversity of beta-thalassaemias (β-thal). The results demonstrate that the pools of free globin chains in red blood cells were correlating with the severity of the disease in patients with different phenotypes of β-thal. The mechanism and the regulation of synthesis of free globin chains pool in a normal individual and in patients with different β-thal phenotypes could arise from several mechanisms which will require further investigation. The role of the free globin pool in patients with β-thal for development of novel therapeutic approaches based on these potential targets requires further investigation. Pertinent biomarkers improves the diagnosis of the β-thal, especially in low-income countries where they are most common and allows more effective therapeutic intervention leading to more successful therapeutic outcome.