Publication: Calpastatin reduces methamphetamine-induced induction in c-Jun phosphorylation, Bax and cell death in neuroblastoma SH-SY5Y cells
Issued Date
2012-01-06
Resource Type
ISSN
18727972
03043940
03043940
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2-s2.0-84855200580
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Mahidol University
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SCOPUS
Bibliographic Citation
Neuroscience Letters. Vol.506, No.1 (2012), 7-11
Suggested Citation
Jirapa Chetsawang, Wilasinee Suwanjang, Nipawan Pirompul, Piyarat Govitrapong, Banthit Chetsawang Calpastatin reduces methamphetamine-induced induction in c-Jun phosphorylation, Bax and cell death in neuroblastoma SH-SY5Y cells. Neuroscience Letters. Vol.506, No.1 (2012), 7-11. doi:10.1016/j.neulet.2011.10.021 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15132
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Title
Calpastatin reduces methamphetamine-induced induction in c-Jun phosphorylation, Bax and cell death in neuroblastoma SH-SY5Y cells
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Abstract
Methamphetamine (METH) is one of the most commonly abused substances in today's society. Many studies have shown that the process of cell death induced by METH involves with the reception of death signals, an increase in pro-apoptotic proteins (Bax) and an activation of cysteine protease death pathway. The objective of this study was to investigate the neuroprotective effects of calpastatin against METH-induced toxicity in SH-SY5Y neuroblastoma cells by observing cell viability, phosphorylation of transcription factor, c-Jun (phospho-c-Jun) and levels of Bax and Bcl-2. The results showed that METH significantly decreased cell viability in SH-SY5Y cultured cells. Conversely, increase in phospho-c-Jun and Bax/Bcl-2 ratio was observed in METH-treated cells. Calpastatin reversed the toxic effect of METH by increasing cell viability, reducing phospho-c-Jun and Bax/Bcl-2 ratio in METH-treated cells. These results indicated that calpastatin has the capacity to reverse an activation of the death process in METH-treated dopaminergic cell lines. © 2011 Elsevier Ireland Ltd.