Publication: A predominance of clade 17 Candida albicans isolated from hemocultures in a tertiary care hospital in Thailand
Issued Date
2019-01-01
Resource Type
ISSN
1664302X
Other identifier(s)
2-s2.0-85069167174
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Frontiers in Microbiology. Vol.10, No.JUN (2019)
Suggested Citation
Linh Thi Truc Pham, Sujiraphong Pharkjaksu, Piriyaporn Chongtrakool, Kamol Suwannakarn, Popchai Ngamskulrungroj A predominance of clade 17 Candida albicans isolated from hemocultures in a tertiary care hospital in Thailand. Frontiers in Microbiology. Vol.10, No.JUN (2019). doi:10.3389/fmicb.2019.01194 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/51122
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
A predominance of clade 17 Candida albicans isolated from hemocultures in a tertiary care hospital in Thailand
Other Contributor(s)
Abstract
Copyright © 2019 Pham, Pharkjaksu, Chongtrakool, Suwannakarn and Ngamskulrungroj. Candida albicans is one of the most common human fungal pathogens. Candidemia has significant mortality globally. No epidemiological study of C. albicans based on multilocus sequence typing (MLST) has been conducted in Thailand. Therefore, MLST was used to study the molecular epidemiology of C. albicans blood strains in a large Thai teaching hospital. In vitro virulence phenotypes and antifungal susceptibility testing by broth microdilution were also conducted. Forty-six C. albicans blood strains from 37 patients were collected from the Department of Microbiology, Siriraj Hospital, in 2016 and 2017. Most patients (71.8%) were more than 60 years old, and the case fatality rate was 54.8%. The male-to-female ratio was 5:3. Thirty-four diploid sequence types (DSTs), including six new DSTs, were identified, with DST2514 (8.7%) and DST2876 (8.7%) as the most common DSTs. Strains were clustered into nine clades. Unlike other studies of C. albicans blood strains in Asia, clade 17 was the most common (13 strains, 28.3%). Sequential allelic changes were evident in sequential strains from one patient. All strains produced phospholipase and hemolysin, while none produced proteinase. The ability to form biofilm was found in 82.6% of the strains. Clade 17 strains showed significantly stronger hemolytic activity than non-clade 17 strains (69.2% versus 27.3%; p = 0.022). However, no significant association existed between clades and patient mortalities. All were susceptible or wild type to anidulafungin (MIC range = 0.015-0.12 and GM = 0.030), micafungin (MIC range = ≤ 0.008-0.015 and GM = 0.008), caspofungin (MIC range = 0.008-0.12 and GM = 0.036), and amphotericin B (MIC range = 0.25-0.5 and GM = 0.381). Only one strain was resistant to voriconazole (MIC range = ≤ 0.008 to ≥ 8 and GM = 0.010) and fluconazole (MIC range = 0.12-16 and GM = 0.398). In conclusion, a high prevalence of clade 17 C. albicans blood strains was found in Thailand, in contrast to other Asian countries. This unique finding might be explained by the strong hemolytic activity that is required for bloodstream infection of C. albicans.