Publication: Persistent expression of shrimp-virus antigens in two insect cell lines challenged with two shrimp viruses
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Issued Date
2009-06-01
Resource Type
ISSN
0388788X
Other identifier(s)
2-s2.0-69949173983
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Fish Pathology. Vol.44, No.2 (2009), 86-93
Suggested Citation
Anuwat Ton, Nipaporn Kanthong, Warachin Gangnonngiw, Siriporn Sriurairatana, Sukathida Ubol, Timothy W. Flegel Persistent expression of shrimp-virus antigens in two insect cell lines challenged with two shrimp viruses. Fish Pathology. Vol.44, No.2 (2009), 86-93. doi:10.3147/jsfp.44.86 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/27011
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Title
Persistent expression of shrimp-virus antigens in two insect cell lines challenged with two shrimp viruses
Abstract
Experiments with crustacean viruses are hampered by lack of susceptible continuous cell lines. To overcome this problem, immortal mosquito and lepidopteran cell lines were both separately challenged with a shrimp DNA virus (white spot syndrome virus: WSSV, = PRDV) and RNA virus (yellow head virus: YHV) followed by serial, split-passage with immunohistochemical monitoring by confocal laser microscopy using labeled monoclonal antibodies to shrimp viral antigens. Stable, immortal cultures with 100% of the cells expressing shrimp-virus antigens were obtained, although the infected cells appeared grossly normal by phase contrast microscopy. Nor did they show any ultrastructural modifications characteristic of the challenge viruses. These persistently-expressing insect cell cultures were stable and could be continuously passaged, stored and revived as required. Since disparate viruses and insect cells were used, this appears to be a generic process that may be applicable to other shrimp viruses as well. © 2009 The Japanese Society of Fish Pathology.