Publication: Determination of discriminatory power of genetic markers used for genotyping giardia duodenalis
Issued Date
2011-07-01
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ISSN
01251562
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2-s2.0-80054913809
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.42, No.4 (2011), 764-771
Suggested Citation
Suradej Siripattanapipong, Saovanee Leelayoova, Mathirut Mungthin, Rc Andrew Thompson, Parima Boontanom, Wilai Saksirisamphant, Peerapan Tan-Ariya Determination of discriminatory power of genetic markers used for genotyping giardia duodenalis. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.42, No.4 (2011), 764-771. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/12438
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Title
Determination of discriminatory power of genetic markers used for genotyping giardia duodenalis
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Abstract
Small subunit ribosomal DNA (SSU-rDNA), glutamate dehydrogenase (gdh), β-giardin, triosephosphate isomerase (tpi), and elongation factor 1-alpha (ef1-α) genes are useful genetic markers for genotypic analysis of the intestinal protozoan, Giardia duodenalis (syn. G. lamblia, G. intestinalis), the cause of enteric disease in humans. To quantitatively compare the discriminatory power of these loci, 43 fecal samples were collected from central, northern and eastern Thailand and G. duodenalis specimens were analyzed using PCR-based genotyping and subcloning methods. Approximately equal prevalence of assemblage A (21) and B (22) were present among these populations. Analysis of Simpson's index and Wallace coefficient values from assemblage B isolates together with the data obtained from GenBank showed that the combination of two loci provides a higher discrimination power for subgenotyping G. duodenalis than using any single locus.