Publication: Monoclonal antibodies against a phencyclidine derivative are used to investigate protein-ligand interactions
Issued Date
1993-10-15
Resource Type
ISSN
09224106
Other identifier(s)
2-s2.0-0027425983
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Mahidol University
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SCOPUS
Bibliographic Citation
European Journal of Pharmacology: Molecular Pharmacology. Vol.247, No.2 (1993), 209-213
Suggested Citation
Stefano O. Casalotti, Alan P. Kozikowski, Abdul Fauq, Werner Tückemantel, Karl E. Krueger Monoclonal antibodies against a phencyclidine derivative are used to investigate protein-ligand interactions. European Journal of Pharmacology: Molecular Pharmacology. Vol.247, No.2 (1993), 209-213. doi:10.1016/0922-4106(93)90079-O Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22804
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Title
Monoclonal antibodies against a phencyclidine derivative are used to investigate protein-ligand interactions
Abstract
Monoclonal antibodies against the irreversible alkylator N-ethyl-1-[2-(4-isothiocyanothienyl)]cyclohexylamine (ITCE) of the 1-[1-(2-thienyl)cyclohexyl]piperidine (TCP) binding site of the N-methyl-d-aspartate receptor were raised. Each antibody was characterized in a competition enzyme-linked immunosorbent assay (ELISA) with a range of TCP analogs. It was found that each monoclonal antibody has a different affinity profile for the various TCP analogs. No correlation between the structure of the side chain groups of each compound and the selective affinities of the antibodies could be deduced, indicating that the overall affinity of the antibodies is determined by more than just the sum of the interaction forces with each ligand's functional groups. In addition to the possible identification of endogenous TCP-like compounds these antibodies could be used as a model to study the molecular interaction between drugs and their receptors' active sites. © 1993.