Publication:
Identification of immunodominant cd4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E

Issued Date

2015-02-10

Resource Type

Article

ISSN

19326203

DOI

10.1371/journal.pone.0115582

Other identifier(s)

2-s2.0-84922639329

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

PLoS ONE. Vol.10, No.2 (2015)

Suggested Citation

Silvia Ratto-Kim, Mark S. De Souza, Jeffrey R. Currier, Nicos Karasavvas, John Sidney, Morgane Rolland, Anais Valencia-Micolta, Sirinan Madnote, Alessandro Sette, Sorachai Nitayaphan, Punnee Pitisuttuthum, Jaranit Kaewkungwal, Supachai Rerks-Ngarm, Robert O'Connell, Nelson Michael, Merlin L. Robb, Mary Marovich, Jerome H. Kim Identification of immunodominant cd4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E. PLoS ONE. Vol.10, No.2 (2015). doi:10.1371/journal.pone.0115582 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35202

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Title

Identification of immunodominant cd4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E

Author(s)

Silvia Ratto-Kim
 Mark S. De Souza
 Jeffrey R. Currier
 Nicos Karasavvas
 John Sidney
 Morgane Rolland
 Anais Valencia-Micolta
 Sirinan Madnote
 Alessandro Sette
 Sorachai Nitayaphan
 Punnee Pitisuttuthum
 Jaranit Kaewkungwal
 Supachai Rerks-Ngarm
 Robert O'Connell
 Nelson Michael
 Merlin L. Robb
 Mary Marovich
 Jerome H. Kim

Other Contributor(s)

Walter Reed Army Institute of Research
 Armed Forces Research Institute of Medical Sciences, Thailand
 La Jolla Institute for Allergy and Immunology
 Mahidol University
 Thailand Ministry of Public Health
 National Institutes of Health, Bethesda
 National Institute of Infectious Diseases
 Water Reed Army Institute of Research

Abstract

We performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine epitope mapping of the V2 and C1 responses and the HLA class II restriction. Data showed that there are two CD4+ epitopes contained within the V2 loop: one encompassing the α4β7 integrin binding site (AA179-181) and the other nested between two previously described genetic sieve signatures (AA169, AA181). There was no correlation between the frequencies of CD4+ fine epitope responses and binding antibody.

Keyword(s)

Agricultural and Biological Sciences
 Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/35202

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Scopus 2011-2015