Publication: Cassava root membrane proteome reveals activities during storage root maturation
Issued Date
2016-01-01
Resource Type
ISSN
09189440
Other identifier(s)
2-s2.0-84953361044
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Plant Research. Vol.129, No.1 (2016), 51-65
Suggested Citation
Maliwan Naconsie, Manassawe Lertpanyasampatha, Unchera Viboonjun, Supatcharee Netrphan, Masayoshi Kuwano, Naotake Ogasawara, Jarunya Narangajavana Cassava root membrane proteome reveals activities during storage root maturation. Journal of Plant Research. Vol.129, No.1 (2016), 51-65. doi:10.1007/s10265-015-0761-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41625
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Cassava root membrane proteome reveals activities during storage root maturation
Abstract
© 2015, The Botanical Society of Japan and Springer Japan. Cassava (Manihot esculenta Crantz) is one of the most important crops of Thailand. Its storage roots are used as food, feed, starch production, and be the important source for biofuel and biodegradable plastic production. Despite the importance of cassava storage roots, little is known about the mechanisms involved in their formation. This present study has focused on comparison of the expression profiles of cassava root proteome at various developmental stages using two-dimensional gel electrophoresis and LC-MS/MS. Based on an anatomical study using Toluidine Blue, the secondary growth was confirmed to be essential during the development of cassava storage root. To investigate biochemical processes occurring during storage root maturation, soluble and membrane proteins were isolated from storage roots harvested from 3-, 6-, 9-, and 12-month-old cassava plants. The proteins with differential expression pattern were analysed and identified to be associated with 8 functional groups: protein folding and degradation, energy, metabolism, secondary metabolism, stress response, transport facilitation, cytoskeleton, and unclassified function. The expression profiling of membrane proteins revealed the proteins involved in protein folding and degradation, energy, and cell structure were highly expressed during early stages of development. Integration of these data along with the information available in genome and transcriptome databases is critical to expand knowledge obtained solely from the field of proteomics. Possible role of identified proteins were discussed in relation with the activities during storage root maturation in cassava.