Publication: Development of a colloidal gold nanoparticle-based immunochromatographic strip for the one-step detection of miroestrol and puerarin
Issued Date
2018-11-01
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ISSN
10990801
02693879
02693879
DOI
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2-s2.0-85052482177
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Mahidol University
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SCOPUS
Bibliographic Citation
Biomedical Chromatography. Vol.32, No.11 (2018)
Suggested Citation
Supaluk Krittanai, Tharita Kitisripanya, Orapin Udomsin, Hiroyuki Tanaka, Seiichi Sakamoto, Thaweesak Juengwatanatrakul, Waraporn Putalun Development of a colloidal gold nanoparticle-based immunochromatographic strip for the one-step detection of miroestrol and puerarin. Biomedical Chromatography. Vol.32, No.11 (2018). doi:10.1002/bmc.4330 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45018
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Title
Development of a colloidal gold nanoparticle-based immunochromatographic strip for the one-step detection of miroestrol and puerarin
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Abstract
© 2018 John Wiley & Sons, Ltd. Pueraria candollei or White Kwao Krua (Leguminosae) is an indigenous plant in Thailand which has long been used in Thai traditional medicine. The tuberous root of this plant is widely used for rejuvenation, particularly in elder women. Among the bioactive compounds in P. candollei, miroestrol and puerarin exhibit estrogenic activity. This study aims to develop an immunochromatographic strip (ICS) with a colloidal gold-based detection system for the simultaneous detection of miroestrol and puerarin in a one-step analysis. The developed method is sensitive and specific for the detection of miroestrol and puerarin in raw materials and marketed products. The detection limits of miroestrol and puerarin were 0.15 and 4.5 μg, respectively. In addition, the results from the developed ICS were confirmed with an enzyme-linked immunosorbent assay and presented a good correlation between these two methods. This is the first report on the development of an ICS that can detect miroestrol and puerarin in one step. The developed ICS provides a simplified method for the detection of miroestrol and puerarin in P. candollei and Pueraria spp.