Publication:
Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli

1

Issued Date

2004-04-01

Resource Type

Article

ISSN

01415492

DOI

10.1023/B:BILE.0000023024.27549.6d

Other identifier(s)

2-s2.0-3342984173

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Biotechnology Letters. Vol.26, No.8 (2004), 649-653

Suggested Citation

Boonhiang Promdonkoy, Saradee Warit, Sakol Panyim Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli. Biotechnology Letters. Vol.26, No.8 (2004), 649-653. doi:10.1023/B:BILE.0000023024.27549.6d Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/21205

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Title

Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli

Author(s)

Boonhiang Promdonkoy
 Saradee Warit
 Sakol Panyim

Other Contributor(s)

Thailand National Center for Genetic Engineering and Biotechnology
 Mahidol University

Abstract

Giant catfish growth hormone (gcGH) cDNA was cloned and expressed in E. coli. The expected 20.5 kDa protein corresponded to the mature gcGH and was efficiently expressed. This protein was produced as inclusion bodies and comprised about 20% of total cellular proteins. The recombinant hormone promoted growth when injected intramuscularly or intraperitoneally into goldfish (Carassius auratus) at 0.1 or 1 μg soluble gcGH per g fish body wt per week. In addition, the recombinant gcGH inclusions had growth-promoting activity similar to that of the soluble form when the fish was received either by intraperitoneal injection or by oral administration.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/21205

Collections

Scopus 2001-2005