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Molecular investigation by whole exome sequencing revealed a high proportion of pathogenic variants among Thai victims of sudden unexpected death syndrome

dc.contributor.authorBhoom Suktitipaten_US
dc.contributor.authorSakda Sathirareuangchaien_US
dc.contributor.authorEkkapong Roothumnongen_US
dc.contributor.authorWanna Thongnoppakhunen_US
dc.contributor.authorPurin Wangkiratikanten_US
dc.contributor.authorNutchavadee Vorasanen_US
dc.contributor.authorRungroj Krittayaphongen_US
dc.contributor.authorManop Pithukpakornen_US
dc.contributor.authorWarangkna Boonyapisiten_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-12-21T06:28:11Z
dc.date.accessioned2019-03-14T08:02:27Z
dc.date.available2018-12-21T06:28:11Z
dc.date.available2019-03-14T08:02:27Z
dc.date.issued2017-07-01en_US
dc.description.abstract© 2017 Suktitipat et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Introduction: Sudden unexpected death syndrome (SUDS) is an important cause of death in young healthy adults with a high incident rate in Southeast Asia; however, there are no molecular autopsy reports about these victims. We performed a combination of both a detailed autopsy and a molecular autopsy by whole exome sequencing (WES) to investigate the cause of SUDS in Thai sudden death victims. Materials and methods: A detailed forensic autopsy was performed to identify the cause of death, followed by a molecular autopsy, in 42 sudden death victims who died between January 2015 and August 2015. The coding sequences of 98 SUDS-related genes were sequenced using WES. Potentially causative variants were filtered based on the variant functions annotated in the dbNSFP database. Variants with inconclusive clinical significance evidence in ClinVar were resolved with a variant prediction algorithm, metaSVM, and the frequency data of the variants found in public databases, such as the 1000 Genome Project, ESP6500 project, and the Exome Aggregation Consortium (ExAc) project. Results: Combining both autopsy and molecular autopsy enabled the potential identification of cause of death in 81% of the cases. Among the 25 victims with WES data, 72% (18/25) were found to have potentially causative SUDS mutations. The majority of the victims had at a mutation in the TTN gene (8/18 = 44%), and only one victim had an SCN5A mutation. Conclusions: WES can help to identify the genetic causes in victims of SUDS and may help to further guide investigations into their relatives to prevent additional SUDS victims.en_US
dc.identifier.citationPLoS ONE. Vol.12, No.7 (2017)en_US
dc.identifier.doi10.1371/journal.pone.0180056en_US
dc.identifier.issn19326203en_US
dc.identifier.other2-s2.0-85023632130en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/41490
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85023632130&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleMolecular investigation by whole exome sequencing revealed a high proportion of pathogenic variants among Thai victims of sudden unexpected death syndromeen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85023632130&origin=inwarden_US

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