Publication: Overexpression of Fusarium solani lipase in Pichia pastoris and its application in lipid degradation
Issued Date
2016-09-02
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ISSN
13102818
Other identifier(s)
2-s2.0-84978477038
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Mahidol University
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SCOPUS
Bibliographic Citation
Biotechnology and Biotechnological Equipment. Vol.30, No.5 (2016), 885-893
Suggested Citation
Jinaporn Wongwatanapaiboon, Waraporn Malilas, Chalermchai Ruangchainikom, Gamgarn Thummadetsak, Suphang Chulalaksananukul, Alain Marty, Warawut Chulalaksananukul Overexpression of Fusarium solani lipase in Pichia pastoris and its application in lipid degradation. Biotechnology and Biotechnological Equipment. Vol.30, No.5 (2016), 885-893. doi:10.1080/13102818.2016.1202779 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42930
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Title
Overexpression of Fusarium solani lipase in Pichia pastoris and its application in lipid degradation
Abstract
© 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. Fusarium solani NAN103 lipase was successfully overexpressed in Pichia pastoris using inducible expression system and constitutive expression system under the control of alcohol oxidase 1 promoter (pAOX1) and glyceraldehyde-3-phosphate dehydrogenase promoter (pGAP), respectively. Lipase obtained using the constitutive promoter showed the highest activity of 18.8 U/mg in 3 days of cultivation time. Optimal lipase activity was observed at pH 7.0 and 35 °C using p-nitrophenyl laurate as the substrate. Lipase activity was enhanced by Mn2+, Ba2+, Li+, Ca2+, Ni2+, CHAPS and Triton X-100 but was inhibited by Hg2+, Ag+ and SDS. The addition of 10% v/v of octanol, p-xylene, hexane and isopropanol increased lipase activity. Cultivation of lipase-expressing P. pastoris under pGAP in synthetic wastewater containing 1% w/v palm oil resulted in degradation of 87% of the oil within 72 h. P. pastoris expressing F. solani lipase from constitutive expression system has the potential to be used as an alternative microorganism for lipid degradation.