Publication: Sergentomyia (Neophlebotomus) gemmea, a potential vector of Leishmania siamensis in southern Thailand
Issued Date
2013-07-19
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ISSN
14712334
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2-s2.0-84880335815
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Mahidol University
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SCOPUS
Bibliographic Citation
BMC Infectious Diseases. Vol.13, No.1 (2013)
Suggested Citation
Kobkan Kanjanopas, Suradej Siripattanapipong, Ubolrat Ninsaeng, Atitaya Hitakarun, Somnat Jitkaew, Preecha Kaewtaphaya, Peerapan Tan-ariya, Mathirut Mungthin, Chetsuda Charoenwong, Saovanee Leelayoova Sergentomyia (Neophlebotomus) gemmea, a potential vector of Leishmania siamensis in southern Thailand. BMC Infectious Diseases. Vol.13, No.1 (2013). doi:10.1186/1471-2334-13-333 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/32253
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Title
Sergentomyia (Neophlebotomus) gemmea, a potential vector of Leishmania siamensis in southern Thailand
Abstract
Background: Leishmaniasis, caused by Leishmania siamensis, is an emerging disease in Thailand. Although reported cases have been increasing, epidemiological information of the disease including host and vector aspects is not clearly known. This study was a preliminary survey of the potential vector of L. siamensis in an affected area of leishmaniasis, Trang Province, southern Thailand.Methods: The collection of sandflies was performed around the area where a case of leishmaniasis was reported using CDC light traps. Species of sandfly were identified based on morphological characteristics according to Lewis's key. PCR amplification and sequencing of the heat shock protein 70 gene (hsp70) was used to identify L. siamensis DNA in sandflies.Results: A total of 146 male and female sandflies were collected in the affected areas. Of 71 female sandflies, four species were identified, i.e., Sergentomyia (Neophlebotomus) gemmea, S. (Neophlebotomus) iyengari, S. (Parrotomyia) barraudi and Phlebotomus (Anaphlebotomus) stantoni. Among these species, S. (Neophlebotomus) gemmea was the most predominant species in all areas. DNA of L. siamensis was identified in S. (Neophlebotomus) gemmea. Nucleotide sequences of PCR products using DNA extracted from S. (Neophlebotomus) gemmea showed 99.8% identity to L. siamensis.Conclusion: S. (Neophlebotomus) gemmea might be a potential vector of L. siamensis in an affected area, Trang Province, southern Thailand. However further studies are needed to prove whether these sandflies can be natural vectors of leishmaniasis. © 2013 Kanjanopas et al.; licensee BioMed Central Ltd.