Publication: Opisthorchis viverrini: Analysis of the sperm-specific rhophilin associated tail protein 1-like
Issued Date
2014-01-01
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ISSN
18736254
0001706X
0001706X
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2-s2.0-84906345871
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Mahidol University
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SCOPUS
Bibliographic Citation
Acta Tropica. Vol.140, (2014), 34-40
Suggested Citation
Sitthichon Rattanachan, Rudi Grams, Smarn Tesana, Peter M. Smooker, Suksiri Vichasri Grams Opisthorchis viverrini: Analysis of the sperm-specific rhophilin associated tail protein 1-like. Acta Tropica. Vol.140, (2014), 34-40. doi:10.1016/j.actatropica.2014.08.002 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33142
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Title
Opisthorchis viverrini: Analysis of the sperm-specific rhophilin associated tail protein 1-like
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Abstract
Concurrent deficiency of rhophilin associated tail protein (ROPN1) and ROPN1-like (ROPN1L) in mice causes structural abnormalities and immotility of sperm and thereby infertility. In the present research, ROPN1L of the human liver fluke Opisthorchis viverrini was molecularly characterized and showed unexpected potential as a diagnostic tool. ROPN1L transcripts were detected in 2-week-old juveniles by RT-PCR. Immunohistochemical analysis of the adult worm localized the protein in testis lobes, seminal vesicle and receptacle and immunoelectron microscopic analysis revealed its location on the tail of spermatozoa. Interestingly, sera of experimentally infected hamsters and sera of individuals suffering from opisthorchiasis showed reactivity to recombinant OvROPN1L (rOvROPN1L). The protein shows modest conservation to the human homolog at 47.2% sequence identity and a mouse anti-rOvROPN1L antiserum was not reactive with sperm protein extracts from hamsters, mice and rats. Unsurprisingly, conservation is higher in trematodes, e.g. 78.4% and 71.2% identity to Fasciola gigantica and Schistosoma haematobium, respectively and evaluation of diagnostic specificity is required using sera of individuals suffering from different trematodiases in Thailand. © 2014 Elsevier B.V.