Publication: Mutation in the rpoB gene of the rifampicin resistant M. avium complex strains from Thailand.
Issued Date
2006-12-01
Resource Type
ISSN
01251562
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2-s2.0-34447530713
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Mahidol University
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SCOPUS
Bibliographic Citation
The Southeast Asian journal of tropical medicine and public health. Vol.37 Suppl 3, (2006), 165-173
Suggested Citation
Oranuch Kongpetchsatit, Wanchai Phatihattakorn, Yuvadee Mahakunkijcharoen, Boonchuay Eampokalarp, Jirakan Boonyasopun, Pongrama Ramasoota Mutation in the rpoB gene of the rifampicin resistant M. avium complex strains from Thailand.. The Southeast Asian journal of tropical medicine and public health. Vol.37 Suppl 3, (2006), 165-173. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23404
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Title
Mutation in the rpoB gene of the rifampicin resistant M. avium complex strains from Thailand.
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Abstract
Forms of mutation never before described in the rpoB gene are reported for a sample of 20 rifampicin-resistant Mycobacterium avium Complex (MAC) strains isolated from AIDS patients in Thailand. All strains were analyzed by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and polymerase chain reaction-DNA sequencing (PCR-DNA sequencing). Sequence analysis of these strains revealed that only one strain (5%) has missense mutation at Lys-626 (Thr) and the rest of the strains had 15 different silent mutations within a 542 bp region of the rpoB gene. Five strains (25%) had a silent mutation at only one position, 7 (35%) at 2 positions, 7 (35%) at 3 positions, and 1 (5%) at 7 positions. The silent mutation at the Ala-630 codon occurred in the largest proportion of the strains (15 strains, 75%), followed by the Val-581 in 8 strains (40%), Tyr-578 and Thr-600 in 4 strains (20%), and Gly-597 in 3 strains (15%). This investigation demonstrates that mutation in the rpoB gene of MAC strains from Thailand are more varied than previously reported for RIF MAC strains. PCR-SSCP screening clearly separated RIFr strains from rifampicin-susceptible (RIFs) strains.