Publication: Identification of Escherichia coli and related enterobacteriaceae and examination of their phenotypic antimicrobial resistance patterns: A pilot study at a wildlife-livestock interface in Lusaka, Zambia
Issued Date
2021-03-01
Resource Type
ISSN
20796382
Other identifier(s)
2-s2.0-85102130057
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Mahidol University
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SCOPUS
Bibliographic Citation
Antibiotics. Vol.10, No.3 (2021), 1-17
Suggested Citation
Emmanuel Kabali, Girja Shanker Pandey, Musso Munyeme, Penjaninge Kapila, Andrew Nalishuwa Mukubesa, Joseph Ndebe, John Bwalya Muma, Charles Mubita, Walter Muleya, Elizabeth Muligisa Muonga, Shuya Mitoma, Bernard Mudenda Hang’ombe, Anuwat Wiratsudakul, Mai Thi Ngan, Eslam Elhanafy, Hala El Daous, Nguyen Thi Huyen, Wataru Yamazaki, Tamaki Okabayashi, Maiku Abe, Junzo Norimine, Satoshi Sekiguchi Identification of Escherichia coli and related enterobacteriaceae and examination of their phenotypic antimicrobial resistance patterns: A pilot study at a wildlife-livestock interface in Lusaka, Zambia. Antibiotics. Vol.10, No.3 (2021), 1-17. doi:10.3390/antibiotics10030238 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76263
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Title
Identification of Escherichia coli and related enterobacteriaceae and examination of their phenotypic antimicrobial resistance patterns: A pilot study at a wildlife-livestock interface in Lusaka, Zambia
Author(s)
Emmanuel Kabali
Girja Shanker Pandey
Musso Munyeme
Penjaninge Kapila
Andrew Nalishuwa Mukubesa
Joseph Ndebe
John Bwalya Muma
Charles Mubita
Walter Muleya
Elizabeth Muligisa Muonga
Shuya Mitoma
Bernard Mudenda Hang’ombe
Anuwat Wiratsudakul
Mai Thi Ngan
Eslam Elhanafy
Hala El Daous
Nguyen Thi Huyen
Wataru Yamazaki
Tamaki Okabayashi
Maiku Abe
Junzo Norimine
Satoshi Sekiguchi
Girja Shanker Pandey
Musso Munyeme
Penjaninge Kapila
Andrew Nalishuwa Mukubesa
Joseph Ndebe
John Bwalya Muma
Charles Mubita
Walter Muleya
Elizabeth Muligisa Muonga
Shuya Mitoma
Bernard Mudenda Hang’ombe
Anuwat Wiratsudakul
Mai Thi Ngan
Eslam Elhanafy
Hala El Daous
Nguyen Thi Huyen
Wataru Yamazaki
Tamaki Okabayashi
Maiku Abe
Junzo Norimine
Satoshi Sekiguchi
Other Contributor(s)
Graduate School of Medicine and Veterinary Medicine
University of Zambia
National Institute of Veterinary Research Hanoi
Vietnam National University of Agriculture
Benha University
Hokkaido University
Mahidol University
Kyoto University
University of Miyazaki
Eden University
Zambia Medicines Regulatory Authority
University of Zambia
National Institute of Veterinary Research Hanoi
Vietnam National University of Agriculture
Benha University
Hokkaido University
Mahidol University
Kyoto University
University of Miyazaki
Eden University
Zambia Medicines Regulatory Authority
Abstract
A cross-sectional study was used to identify and assess prevalence and phenotypic antimicrobial resistance (AMR) profiles of Escherichia coli and other enterobacteria isolated from healthy wildlife and livestock cohabiting at a 10,000 acres game ranch near Lusaka, Zambia. Purposive sampling was used to select wildlife and livestock based on similarities in behavior, grazing habits and close interactions with humans. Isolates (n = 66) from fecal samples collected between April and August 2018 (n = 84) were examined following modified protocols for bacteria isolation, biochemical identification, molecular detection, phylogenetic analysis, and antimicrobial susceptibility testing by disc diffusion method. Data were analyzed using R software, Genetyx ver.12 and Mega 6. Using Applied Profile Index 20E kit for biochemical identification, polymerase chain reaction assay and sequencing, sixty-six isolates were identified to species level, of which Escherichia coli (72.7%, 48/66), E. fergusonii (1.5%, 1/66), Shigella sonnei (22.7%, 14/66), Sh. flexinerri (1.5%, 1/66) and Enterobacteriaceae bacterium (1.5%, 1/66), and their relationships were illustrated in a phylogenetic tree. Phenotypic antimicrobial resistance or intermediate sensitivity expression to at least one antimicrobial agent was detected in 89.6% of the E. coli, and 73.3% of the Shigella isolates. The E. coli isolates exhibited the highest resistance rates to ampicillin (27%), ceftazidime (14.3%), cefotaxime (9.5%), and kanamycin (9.5%). Multidrug resistance (MDR) was detected in 18.8% of E. coli isolates while only 13.3% Shigella isolates showed MDR. The MDR was detected among isolates from impala and ostrich (wild animals in which no antimicrobial treatment was used), and in isolates from cattle, pigs, and goats (domesticated animals). This study indicates the possible transmission of drug-resistant microorganisms between animals cohabiting at the wildlife-livestock interface. It emphasizes the need for further investigation of the role of wildlife in the development and transmission of AMR, which is an issue of global concern.