Publication: Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosis
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Issued Date
2021-09-01
Resource Type
ISSN
18783511
12019712
12019712
Other identifier(s)
2-s2.0-85111822762
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Infectious Diseases. Vol.110, (2021), 75-82
Suggested Citation
Phyu Thwe Soe, Jariya Hanthamrongwit, Chutiphon Saelee, Soe Paing Kyaw, Prasong Khaenam, Saradee Warit, Nusara Satproedprai, Surakameth Mahasirimongkol, Hideki Yanai, Patchanee Chootong, Chaniya Leepiyasakulchai Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosis. International Journal of Infectious Diseases. Vol.110, (2021), 75-82. doi:10.1016/j.ijid.2021.07.033 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/77915
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Title
Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosis
Abstract
Objective: To elucidate the antigenic potential of dormancy-associated antigens Rv2659c and Rv3128c of Mycobacterium tuberculosis by examining the persistence of specific IgG and IgA memory B cells (MBCs) among patients with active tuberculosis (TB), household contacts with latent tuberculosis (LTBI), and an endemic healthy control group. Methods: Fresh peripheral blood mononuclear cells from the three study groups were used to enumerate the numbers of IgG and IgA MBCs specific to recombinant protein Rv2659c and Rv3128c by ELISpot assay. The composition of MBC subsets IgA+ and IgG + was analyzed by flow cytometry. Results: The number of IgA MBCs specific to antigen Rv2659c was significantly higher in the LTBI group than the TB group. In contrast, no significant difference was found in IgA or IgG MBCs against antigen Rv3128c. The number of IgA+ MBCs was significantly higher than that of IgG+ MBCs in the classical MBC subset of the LTBI group. Conclusion: The results indicated that the dormancy-associated antigen Rv2659c induced an IgA MBCs response in individuals with latent TB, and IgA+ classical MBCs formed a major portion of the MBCs subset. This new knowledge will be beneficial for the development of novel TB vaccines and their control of latent TB.