Publication: Effect of exogenous calcium on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis (rubber tree) friable calli
Issued Date
2000-01-01
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ISSN
07217714
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2-s2.0-0033800862
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Mahidol University
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SCOPUS
Bibliographic Citation
Plant Cell Reports. Vol.19, No.9 (2000), 851-855
Suggested Citation
P. Montoro, N. Teinseree, W. Rattana, P. Kongsawadworakul, N. Michaux-Ferriere Effect of exogenous calcium on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis (rubber tree) friable calli. Plant Cell Reports. Vol.19, No.9 (2000), 851-855. doi:10.1007/s002990000208 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/25826
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Title
Effect of exogenous calcium on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis (rubber tree) friable calli
Abstract
The influence of CaCl2was investigated on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis friable calli which are usually proliferated on maintenance medium (MM) containing 9 mM CaCl2.Five A. tumefaciens strains (C58pMP90, C58pGV2260, AGL1, LBA4404 and EHA 105) and two binary vectors (pGIN and pCAMBIA2301) were tested and the strain EHA105pC2301 was selected to conduct further experiments. The calli were precultured on MM containing a range of CaCl2concentrations, then inoculated with Agrobacterium suspension. Transfer of friable calli from MM containing 9 mM CaCl2to calcium-free medium significantly enhanced the transient β-glucuronidase activity. Interestingly, the use of calcium-free Agrobacterium resuspension medium to inoculate friable calli again dramatically increased the transformation efficiency. Induction of Agrobacterium's virulence with acetosyringone remained an important factor to stimulate transformation.