Publication: Rapid and sensitive detection of shrimp yellow head virus using loop-mediated isothermal amplification and a colorogenic nanogold hybridization probe
Issued Date
2012-11-19
Resource Type
ISSN
18790984
01660934
01660934
Other identifier(s)
2-s2.0-84869067382
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.186, No.1-2 (2012), 36-42
Suggested Citation
Wansadaj Jaroenram, Narong Arunrut, Wansika Kiatpathomchai Rapid and sensitive detection of shrimp yellow head virus using loop-mediated isothermal amplification and a colorogenic nanogold hybridization probe. Journal of Virological Methods. Vol.186, No.1-2 (2012), 36-42. doi:10.1016/j.jviromet.2012.08.013 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/14242
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Title
Rapid and sensitive detection of shrimp yellow head virus using loop-mediated isothermal amplification and a colorogenic nanogold hybridization probe
Abstract
Salt-induced self-aggregation of gold nanoparticles (AuNP) carrying unisense ssDNA probes can be prevented specifically by complementary DNA. Loop-mediated isothermal amplification (LAMP) can amplify DNA rapidly. Here, the two techniques were combined to detect yellow head virus (YHV). The LAMP-AuNP method required 60. min for LAMP and 5. min for hybridization of LAMP products to an AuNP-labeled ssDNA probe followed by salt induced probe-particle aggregation to visualize color development. The detection sensitivity of the method was comparable to that of the commercial IQ2000™ nested RT-PCR but only required ∼65. min to produce a result, and did not cross-detect other shrimp viruses. As the LAMP-AuNP protocol only requires a heating block, it offers opportunities for rapid detection of YHV. © 2012 Elsevier B.V.