Publication: Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs
Issued Date
2018-01-01
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ISSN
13477439
09167250
09167250
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2-s2.0-85057116840
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Veterinary Medical Science. Vol.80, No.11 (2018)
Suggested Citation
Naoki Koike, Thi Ngan Mai, Mamoru Shirai, Meiko Kubo, Kazuhiro Hata, Nobuyuki Marumoto, Shinji Watanabe, Yosuke Sasaki, Shuya Mitoma, Kosuke Notsu, Tamaki Okabayashi, Anuwat Wiratsudakul, Emmanuel Kabali, Junzo Norimine, Satoshi Sekiguchi Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs. Journal of Veterinary Medical Science. Vol.80, No.11 (2018). doi:10.1292/jvms.18-0132 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/47458
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Title
Detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs
Abstract
© 2018, Japanese Society of Veterinary Science. All rights reserved. The purpose of this study was to detect porcine epidemic diarrhea virus (PEDV) subclinically infected pigs shipped from non-case farms to slaughterhouses. Systematic sampling was conducted at two slaughterhouses. A total of 1,556 blood samples were collected from 80 case and non-case farms from pigs over 6 months old. Blood samples were centrifuged to obtain sera. Serial serum dilutions were subjected to serological examination for PEDV presence using Neutralization test (NT). The cut-off titer was set at titer of 1:2 dilution and farms with at least one positive sample in duplicate were classified as PED-positive farms. Several non-case farms (9.4%, 6/64) and 100% (16/16) of the case farms were indeed positive for PEDV. The proportion of seropositive animals from case farms was 63.7%, significantly different from that of non-case farms (4.3%, P<0.05). In both case and non-case farms, the proportion of seropositive animals in farrow-to-finish farms was significantly higher than in wean-to-finish farms (P<0.05). Seropositive animals in non-case farms were detected by NT in a sero-survey by sampling at slaughterhouses. Therefore, subclinically infected pigs should be considered prior to shipment.