Publication: Exposure of Acinetobacter baylyi ADP1 to the biocide chlorhexidine leads to acquired resistance to the biocide itself and to oxidants
Issued Date
2011-02-01
Resource Type
ISSN
14602091
03057453
03057453
Other identifier(s)
2-s2.0-78651416502
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Antimicrobial Chemotherapy. Vol.66, No.2 (2011), 319-322
Suggested Citation
Mayuree Fuangthong, Mudcharee Julotok, Worasamon Chintana, Kristin Kuhn, Surawach Rittiroongrad, Paiboon Vattanaviboon, Skorn Mongkolsuk Exposure of Acinetobacter baylyi ADP1 to the biocide chlorhexidine leads to acquired resistance to the biocide itself and to oxidants. Journal of Antimicrobial Chemotherapy. Vol.66, No.2 (2011), 319-322. doi:10.1093/jac/dkq435 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12693
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Title
Exposure of Acinetobacter baylyi ADP1 to the biocide chlorhexidine leads to acquired resistance to the biocide itself and to oxidants
Abstract
Objectives: The aim of this study was to determine the effect of exposure to sublethal concentrations of chlorhexidine on oxidative stress protection by Acinetobacter baylyi ADP1. Methods: ADP1 cultures were exposed to sublethal concentrations of chlorhexidine prior to being challenged with lethal concentrations of chlorhexidine itself and by oxidants. Oxidant-sensitive dyes and a flow cytometer were used to measure the formation of reactive oxygen species. The role of efflux pumps in chlorhexidine resistance was investigated using a specific inhibitor. Results: Exposure of ADP1 to low concentrations of chlorhexidine induced adaptive and cross-protective responses to chlorhexidine and oxidants (H 2 O 2 and a superoxide anion generator), respectively. Chlorhexidine treatment of ADP1 resulted in the formation of H 2 O 2 and superoxide anions that are probably responsible for the cross-protection against oxidants. Conclusions: Exposure of ADP1 to sublethal concentrations of chlorhexidine confers inducible resistance to lethal concentrations of chlorhexidine itself and to oxidants. An important link was demonstrated between exposure to a biocide and the gaining of resistance to both the biocide and oxidative stress. © The Author 2010. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.