Publication: Rapid simultaneous determination of four indole compounds in dietary supplements by micellar electrokinetic chromatography with a dilute and shoot step
Issued Date
2016-01-21
Resource Type
ISSN
17599679
17599660
17599660
Other identifier(s)
2-s2.0-84954170103
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Analytical Methods. Vol.8, No.3 (2016), 637-643
Suggested Citation
Apichai Phonchai, Prapin Wilairat, Rattikan Chantiwas Rapid simultaneous determination of four indole compounds in dietary supplements by micellar electrokinetic chromatography with a dilute and shoot step. Analytical Methods. Vol.8, No.3 (2016), 637-643. doi:10.1039/c5ay02434j Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43359
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Rapid simultaneous determination of four indole compounds in dietary supplements by micellar electrokinetic chromatography with a dilute and shoot step
Other Contributor(s)
Abstract
© 2016 The Royal Society of Chemistry. A simple micellar electrokinetic chromatography (MEKC) method with UV detection was developed for the simultaneous determination of indole-3-carbinol, indole-3-acetonitrile, indole-3-acetic acid and 3,3′-diindolylmethane. These compounds are potentially used in cancer prevention. Investigation of solvent effects (methanol and dimethylformamide) on MEKC analysis was carried out. A dilute and shoot strategy was used for sample preparation to reduce the time required for multiple steps such as solvent evaporation. The final conditions were electrokinetic injection for 3.0 s at 423 V cm-1and 20.0 mM borate buffer (pH 9.00) containing 20.0 mM SDS. Analysis was rapid, achieved in less than 4.5 min. Linear calibration curves for the indole compounds in the range 5-200 μg mL-1(r2> 0.999) were obtained. Intra- and inter-day precisions were 2.0-7.9% RSD, with LOQs in the range of 1.5-4.0 μg mL-1and recoveries in the range of 90-110% (n = 5).