Publication: Characterization of AvaR1, a butenolide-autoregulator receptor for biosynthesis of a Streptomyces hormone in Streptomyces avermitilis
Issued Date
2016-11-01
Resource Type
ISSN
14320614
01757598
01757598
Other identifier(s)
2-s2.0-84982245086
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Applied Microbiology and Biotechnology. Vol.100, No.22 (2016), 9581-9591
Suggested Citation
Suandi Pratama Sultan, Shigeru Kitani, Kiyoko T. Miyamoto, Hiroyuki Iguchi, Tokitaka Atago, Haruo Ikeda, Takuya Nihira Characterization of AvaR1, a butenolide-autoregulator receptor for biosynthesis of a Streptomyces hormone in Streptomyces avermitilis. Applied Microbiology and Biotechnology. Vol.100, No.22 (2016), 9581-9591. doi:10.1007/s00253-016-7781-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42925
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Characterization of AvaR1, a butenolide-autoregulator receptor for biosynthesis of a Streptomyces hormone in Streptomyces avermitilis
Other Contributor(s)
Abstract
© 2016, Springer-Verlag Berlin Heidelberg. Streptomyces hormones, sometimes called as autoregulators, are important signaling molecules to trigger secondary metabolism across many Streptomyces species. We recently identified a butenolide-type autoregulator (termed avenolide) as a new class of Streptomyces hormone from Streptomyces avermitilis that produces important anthelmintic agent avermectin. Avenolide triggers the production of avermectin with minimum effective concentration of nanomolar. Here, we describe the characterization of avaR1 encoding an avenolide receptor in the regulation of avermectin production and avenolide biosynthesis. The disruption of avaR1 resulted in transcriptional derepression of avenolide biosynthetic gene with an increase in avenolide production, with no change in the avermectin production profile. Moreover, the avaR1 mutant showed increased transcription of avaR1. Together with clear DNA-binding capacity of AvaR1 toward avaR1 upstream region, it suggests that AvaR1 negatively controls the expression of avaR1 through the direct binding to the promoter region of avaR1. These findings revealed that the avenolide receptor AvaR1 functions as a transcriptional repressor for avenolide biosynthesis and its own synthesis.