Publication: High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions
Issued Date
2013-06-01
Resource Type
ISSN
18619541
10016538
10016538
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2-s2.0-84879786609
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Mahidol University
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SCOPUS
Bibliographic Citation
Chinese Science Bulletin. Vol.58, No.16 (2013), 1919-1930
Suggested Citation
Sarunyaporn Maksup, Kanyaratt Supaibulwatana, Gopalan Selvaraj High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions. Chinese Science Bulletin. Vol.58, No.16 (2013), 1919-1930. doi:10.1007/s11434-013-5726-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/32825
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Title
High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions
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Abstract
Rice (Oryza sativa L.) is important to food security and is also an excellent model plant for numerous cereal crops. A functional genomics study in rice includes characterization of the expression dynamics of genes by quantitative real-time PCR (qPCR) analysis; this is a significant key for developing rice varieties that perform well in the face of adverse climate change. The qPCR analysis requires the use of appropriate reference genes in order to make any quantitative interpretations meaningful. Here, the new potential reference genes were selected from a huge public database of rice microarray experiments. The expression stability of 14 candidates and 4 conventional reference genes was validated by geNormPLUSand NormFinder software. Seven candidates are superior to the conventionally used reference genes in qPCR and three genes can be used reliably for quantitating the expression of genes involved in abiotic stress responses. These high-quality references EP (LOC_Os05g08980), HNR (LOC_Os01g71770), and TBC (LOC_Os09g34040) worked very well in three indica genotypes and one japonica genotype. One of indica genotypes including the Jasmine rice, KDML105 developed in Thailand for which no reference genes have been reported until now. © 2013 The Author(s).