Publication: Evaluation of the Virulence of Xanthomonas campestris pv. campestris Mutant Strains Lacking Functional Genes in the OxyR Regulon
Issued Date
2011-08-01
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ISSN
14320991
03438651
03438651
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2-s2.0-80052531397
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Mahidol University
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SCOPUS
Bibliographic Citation
Current Microbiology. Vol.63, No.2 (2011), 232-237
Suggested Citation
Nisanart Charoenlap, Sarinya Buranajitpakorn, Jintana Duang-Nkern, Poommaree Namchaiw, Paiboon Vattanaviboon, Skorn Mongkolsuk Evaluation of the Virulence of Xanthomonas campestris pv. campestris Mutant Strains Lacking Functional Genes in the OxyR Regulon. Current Microbiology. Vol.63, No.2 (2011), 232-237. doi:10.1007/s00284-011-9970-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12010
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Title
Evaluation of the Virulence of Xanthomonas campestris pv. campestris Mutant Strains Lacking Functional Genes in the OxyR Regulon
Abstract
Xanthomonas campestris pv. campestris causes black rot in cruciferous crops. Hydrogen peroxide (H 2 O 2 ) production and accumulation is an important initial response in plant defense against invading microbes. The role of genes involved in the bacterial H 2 O 2 protection system in pathogenicity was evaluated. Mutants of katA (encoding a monofunctional catalase) and, to a lesser extent, katG (encoding a catalase-peroxidase) and oxyR (encoding a H 2 O 2 sensor and a transcription regulator), are hypersensitive to H2O2 treatments that mimic the plant H 2 O 2 burst. These data correlate with the results of pathogenicity testing that show katA, katG, and oxyR mutants are avirulent on a compatible plant. Moreover, exposure to H 2 O 2 (1, 2, and 4 mM) highly induces the expression of genes in the OxyR regulon, including katA, katG, and ahpC. The avirulent phenotype of the oxyR mutant is partly because of its inability to mount an adaptive response upon exposure to an H 2 O 2 burst. Our data provide insights into important roles of a transcription regulator and other genes involved in peroxide stress protection in the virulence of X. campestris pv. campestris. © Springer Science+Business Media, LLC 2011.