Publication: Characterization of two monoclonal antibodies against teguments of adult and schistosomula of Schistosoma japonicum
Issued Date
1991-12-01
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ISSN
0125877X
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2-s2.0-0026321792
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.9, No.2 (1991), 101-106
Suggested Citation
V. Viyanant, P. Sobhon, E. S. Upatham, T. Kusamran, C. Kittigul, W. Panuwatsuk, P. Ardseungnoen, S. Anatawara Characterization of two monoclonal antibodies against teguments of adult and schistosomula of Schistosoma japonicum. Asian Pacific Journal of Allergy and Immunology. Vol.9, No.2 (1991), 101-106. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22089
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Title
Characterization of two monoclonal antibodies against teguments of adult and schistosomula of Schistosoma japonicum
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Abstract
Two monoclonal antibodies (McAbs) were produced from BALB/c mice hyperimmunized with tegumental extract of Schistosoma japonicum (Chinese strain). The two McAbs were characterized with regard to antibody isotype, antigen binding specificity and parasite stage specificity. One McAb, 8G9-5, was identified as IgM, whereas the other McAb, 9E7, was determined to be IgG(2a). Immunoblotting assay indicated that McAb 8G9-5 binds strongly to the band of tegumental antigens of Mr 64 kDa but also binds weakly to other bands at 116, 105, 97, 54, 50, 47 and 45 kDa, whereas 9E7 McAb reacts specifically at Mr 54 kDa. Anatomical localization ot the antigens in the adult worm by indirect immunofluorescence assay indicated that the target epitopes of McAb 8G9-5 are in the intra-tegumental structure, whereas the McAb 9E7 epitope is on the surface membrane. The two McAbs also react at similar sites within the teguments of schistosomula and lung worms.