Publication: The PEI-introduced CS shell/PMMA core nanoparticle for silencing the expression of E6/E7 oncogenes in human cervical cells
Issued Date
2012-10-15
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ISSN
01448617
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2-s2.0-84865639678
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Mahidol University
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SCOPUS
Bibliographic Citation
Carbohydrate Polymers. Vol.90, No.3 (2012), 1323-1329
Suggested Citation
Nattika Saengkrit, Phakorn Sanitrum, Noppawan Woramongkolchai, Somsak Saesoo, Nuttaporn Pimpha, Saowaluk Chaleawlert-Umpon, Tewin Tencomnao, Satit Puttipipatkhachorn The PEI-introduced CS shell/PMMA core nanoparticle for silencing the expression of E6/E7 oncogenes in human cervical cells. Carbohydrate Polymers. Vol.90, No.3 (2012), 1323-1329. doi:10.1016/j.carbpol.2012.06.079 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13939
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Title
The PEI-introduced CS shell/PMMA core nanoparticle for silencing the expression of E6/E7 oncogenes in human cervical cells
Abstract
In this study, we examined the potential of cationic nanoparticle - polyethyleneimine-introduced chitosan shell/poly (methyl methacrylate) core nanoparticles (CS-PEI) for siRNA delivery. Initially, DNA delivery was performed to validate the capability of CS-PEI for gene delivery in the human cervical cancer cell line, SiHa. siRNA delivery were subsequently carried out to evaluate the silencing effect on targeted E6 and E7 oncogenes. Physicochemical properties including size, zeta potential and morphology of CS-PEI/DNA and CS-PEI/siRNA complexes, were analyzed. The surface charges and sizes of the complexes were observed at different N/P ratios. The hydrodynamic sizes of the CS-PEI/DNA and CS-PEI/siRNA were approximately 300-400 and 400-500 nm, respectively. Complexes were positively charged depending on the amount of added CS-PEI. AFM images revealed the mono-dispersed and spherical shapes of the complexes. Gel retardation assay confirmed that CS-PEI nanoparticles completely formed complexes with DNA and siRNA at a N/P ratio of 1.6. For DNA transfection, CS-PEI provided the highest transfection result. Localization of siRNA delivered through CS-PEI was confirmed by differential interference contrast (DIC) confocal imaging. The silencing effect of siRNA specific to HPV 16 E6/E7 oncogene was examined at 18 and 24 h post-transfection. The results demonstrated the capacity of CS-PEI to suppress the expression of HVP oncogenes. © 2012 Elsevier Ltd.