Publication: Further studies on the effect of phosphoenolpyruvate on respiration-dependent calcium transport by rat heart mitochondria
Issued Date
1976-02-16
Resource Type
ISSN
00052728
Other identifier(s)
2-s2.0-0017225177
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
BBA - Bioenergetics. Vol.423, No.2 (1976), 196-202
Suggested Citation
Prakorn Chudapongse Further studies on the effect of phosphoenolpyruvate on respiration-dependent calcium transport by rat heart mitochondria. BBA - Bioenergetics. Vol.423, No.2 (1976), 196-202. doi:10.1016/0005-2728(76)90178-X Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/10841
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Further studies on the effect of phosphoenolpyruvate on respiration-dependent calcium transport by rat heart mitochondria
Author(s)
Other Contributor(s)
Abstract
Phosphoenolpyruvate was found to depress extra oxygen consumption associated with Ca 2+ -induced respiratory jump by rat heart mitochondria. Addition of phosphoenolpyruvate to mitochondria which have accumulated Ca 2+ in the presence of glutamate and inorganic phosphate causes the release of Ca 2+ from mitochondria. The phosphoenolpyruvate-stimulated Ca 2+ efflux can be observed with mitochondria loaded with low initial Ca 2+ concentration (0.12 mM) in the incubation medium. Measurements of mitochondrial H + translocation produced by addition of Ca 2+ to respiring mitochondria show that phosphoenolpyruvate depresses H + ejection and enhances H + uptake by mitochondria. The Ca 2+ -releasing effect of phosphoenolpyruvate was found to be significantly stronger than that produced by rotenone when added to mitochondria loaded with Ca 2+ in the presence of glutamate and inorganic phosphate. Dithiothreitol cannot overcome the effect of phosphoenolpyruvate on mitochondrial Ca 2+ transport. © 1976.