Publication: Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers
Issued Date
2011
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eng
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Mahidol University
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BioMed Central
Bibliographic Citation
BMC Biotechnology. Vol. 11, (2011), 89
Suggested Citation
Khanit Sa-ngiamsuntorn, Adisak Wongkajornsilp, Kanda Kasetsinsombat, Sunisa Duangsa-ard, Lalana Nuntakarn, Suparerk Borwornpinyo, Pravit Akarasereenont, Somchai Limsrichamrern, Suradej Hongeng Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers. BMC Biotechnology. Vol. 11, (2011), 89. doi:10.1186/1472-6750-11-89 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2638
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Title
Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers
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Abstract
Background: The strenuous procurement of cultured human hepatocytes and their short lives have constrained
the cell culture model of cytochrome P450 (CYP450) induction, xenobiotic biotransformation, and hepatotoxicity.
The development of continuous non-tumorous cell line steadily containing hepatocyte phenotypes would
substitute the primary hepatocytes for these studies.
Results: The hepatocyte-like cells have been developed from hTERT plus Bmi-1-immortalized human mesenchymal
stem cells to substitute the primary hepatocytes. The hepatocyte-like cells had polygonal morphology and steadily
produced albumin, glycogen, urea and UGT1A1 beyond 6 months while maintaining proliferative capacity.
Although these hepatocyte-like cells had low basal expression of CYP450 isotypes, their expressions could be
extensively up regulated to 80 folds upon the exposure to enzyme inducers. Their inducibility outperformed the
classical HepG2 cells.
Conclusion: The hepatocyte-like cells contained the markers of hepatocytes including CYP450 isotypes. The high
inducibility of CYP450 transcripts could serve as a sensitive model for profiling xenobiotic-induced expression of
CYP450.