Publication: Quantitation of IgE binding to the chitinase and chitinase-like house dust mite allergens Der p 15 and Der p 18 compared to the major and mid-range allergens
Issued Date
2013-01-01
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ISSN
14230097
10182438
10182438
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2-s2.0-84867481591
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Mahidol University
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SCOPUS
Bibliographic Citation
International Archives of Allergy and Immunology. Vol.160, No.3 (2013), 233-240
Suggested Citation
Belinda J. Hales, Claire E. Elliot, Lee Ying Chai, Leigh J. Pearce, Thitinun Tipayanon, Lee Hazell, Shane Stone, Surapon Piboonpocanun, Wayne R. Thomas, Wendy Anne Smith Quantitation of IgE binding to the chitinase and chitinase-like house dust mite allergens Der p 15 and Der p 18 compared to the major and mid-range allergens. International Archives of Allergy and Immunology. Vol.160, No.3 (2013), 233-240. doi:10.1159/000339760 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31987
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Title
Quantitation of IgE binding to the chitinase and chitinase-like house dust mite allergens Der p 15 and Der p 18 compared to the major and mid-range allergens
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Abstract
© 2012 S. Karger AG, Basel. Background: The prevalence of IgE binding to the group 15 and 18 house dust mite (HDM) allergens of the Dermatophagoides species is reported to be >50% and they are the major allergens of HDM-sensitised dogs. The objective was to quantitate the IgE titres to Der p 15 and Der p 18 and evaluate their importance in human HDM sensitisation compared to the known major and mid-tier allergens. Methods: Der p 15 and Der p 18 were produced in Pichia pastoris, and their structure validated by circular dichroism. IgE binding was measured in 37 Australian HDM-allergic adults using a quantitative DELFIA™ assay. Results: The prevalence of IgE titres to Der p 15 and Der p 18 >0.1 ng/ml was low (38%) and only one subject had a titre >10 ng/ml to either allergen. The mean anti-Der p 15 and Der p 18 titres were 1.2 and 2.6 ng/ml, respectively, i.e. approximately 10- to 20-fold lower than the response to the major Der p 1 and Der p 2 allergens (p < 0.001). The IgE responses to Der p 15 and Der p 18 were lower than the mid-tier allergens Der p 5 and Der p 7 and although they correlated with each other, they did not correlate with titres to either the major or mid-tier allergens. Conclusions: Sensitisation to Der p 15 and Der p 18 makes a minor contribution to anti-HDM IgE titres, and the titres do not correlate with the size of the response to the major allergens.