Publication: Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
Issued Date
2018-01-03
Resource Type
ISSN
07176287
07169760
07169760
Other identifier(s)
2-s2.0-85053732447
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biological Research. Vol.51, No.1 (2018)
Suggested Citation
Bongkoch Turathum, Sittiruk Roytrakul, Chinarat Changsangfa, Morakot Sroyraya, Supita Tanasawet, Yindee Kitiyanant, Kulnasan Saikhun Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis. Biological Research. Vol.51, No.1 (2018). doi:10.1186/s40659-018-0176-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/44835
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
Abstract
© The Author(s) 2017. Background: The domestic cat serves as an animal model for assisted reproductive studies of endangered felid species. To date, there are no data on the protein alterations following cryopreservation of oocytes in felid family. Methods: Immature (germinal vesicle) domestic cat oocytes were vitrified in the vitrification solution containing 35% ethylene glycol, 20% DMSO and 0.5 mM sucrose. The vitrified-warmed oocytes were matured (metaphase II) in vitro and subjected to proteomic analysis using 1DE SDS-PAGE prefractionation combined with LC-MS/MS. Results: A total of 1712 proteins were identified in in vitro matured oocytes. Of the 1712 proteins, 1454 proteins were found in both groups, whereas, 258 proteins were differentially expressed between control and vitrified-warmed groups. In vitrified-warmed oocytes, the missing proteins were membrane and nuclear proteins; whereas, apoptosis and DNA repair proteins were overrepresented. Conclusions: The identified missing and overexpressed proteins in vitrified-warmed oocytes represent potential markers of cryoinjuries and the developmental pathways of oocytes. The findings of differential expressed proteins may contribute to effective ways of proteome analysis of oocyte/embryo quality in order to assess safety of cryopreservation in felid species.