Publication: Mucin production in prostatic adenocarcinoma: A retrospective study of 190 radical prostatectomy and/or core biopsy specimens in department of pathology, siriraj hospital, mahidol university, Thailand
Issued Date
2011-02-01
Resource Type
ISSN
01252208
01252208
01252208
Other identifier(s)
2-s2.0-79952350498
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.94, No.2 (2011), 224-230
Suggested Citation
Siroth Noiwan, Samrerng Rattanarapee Mucin production in prostatic adenocarcinoma: A retrospective study of 190 radical prostatectomy and/or core biopsy specimens in department of pathology, siriraj hospital, mahidol university, Thailand. Journal of the Medical Association of Thailand. Vol.94, No.2 (2011), 224-230. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12642
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Mucin production in prostatic adenocarcinoma: A retrospective study of 190 radical prostatectomy and/or core biopsy specimens in department of pathology, siriraj hospital, mahidol university, Thailand
Author(s)
Other Contributor(s)
Abstract
Background: Diagnoses of prostatic adenocarcinoma are made based on a constellation of architectural, cytological, and ancillary findings, which include intraluminal mucin secretion. Several studies have shown that luminal acid mucin is frequently present in prostatic adenocarcinoma. Immunohistochemical stain (immunostaining) becomes extremely helpful in the diagnosis of prostatic adenocarcinoma, especially in doubtful cases. However, presently, the technique is not available in most hospitals, particularly in the regional or provincial areas. Thus, luminal acid mucin and its detection by histochemical stains may be one of the useful tools in diagnosing prostatic adenocarcinoma. Objective: To determine the percentage of mucin production in prostatic adenocarcinoma from 190 radical prostatectomy and/or core biopsy specimens in the Departmeant of Pathology, Siriraj Hospital, Mahidol University, to compare the percentage of positivity among three different special histochemical stains (mucicarmine, Alcian blue at pH 2.5, and colloidal iron), and to determine the PSA-expression among the prostatic adenocarcinoma that produces mucin by immunohistochemical technique. Material and Method: The present study is a retrospective study of 190 cases that were diagnosed as prostatic adenocarcinoma (with any Gleason's microscopic pattern) from radical prostatectomy and/or core biopsy specimens in Department of Pathology, Siriraj Hospital, Mahidol University between January 2006 and May 2007. All cases were retrieved from a computer filing system of the Department. All H & E slides that contained diagnostic materials were reviewed and stained with all three different special histochemical stains. Positive cases were subsequently studied to find PSA expression by means of immunohistochemical study. Cases with microscopic variants of prostatic adenocarcinoma and metastasis were excluded. Results: One hundred and seventy seven cases (93%) of these prostatic adenocarcinomas were positive for at least one of three different special histochemical stains for acid mucin. Among these, 167 cases (88%) were positive for mucicarmine, 157 (83%) cases were positive for Alcian blue at pH 2.5, and 173 cases (91%) were positive for colloidal iron. All 177 cases were positive for PSA immunohistochemical stain. Conclusion: Intraluminal acid mucin and its detection by special histochemical stains is one of the useful methods (with a high percentage of positivity) in diagnosing prostatic adenocarcinoma in association with other architectural and cytological criteria. Mucicarmine and colloidal iron stains appear to be more useful than Alcian blue at pH 2.5 stain according to their positivity percentage. However, in pathologic practice, mucicarmine stain is more commonly used than colloid iron stain due to the difficulty in preparation and interpretation of the latter. There is no correlation between mucin production and PSA expression in prostatic adenocarcinoma but PSA immunohistochemical stain is still useful to confirm the prostatic origin of poorly differentiated carcinomas or metastatic lesions.