Publication: Genetic characterisation of a whiB7 mutant of a Mycobacterium tuberculosis clinical strain
Issued Date
2015-12-01
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ISSN
22137173
22137165
22137165
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2-s2.0-84952871895
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Global Antimicrobial Resistance. Vol.3, No.4 (2015), 262-266
Suggested Citation
Saradee Warit, Saranya Phunpruch, Chaitas Jityam, Sarinya Jaitrong, Pamaree Billamas, Angkana Chaiprasert, Prasit Palittapongarnpim, Therdsak Prammananan Genetic characterisation of a whiB7 mutant of a Mycobacterium tuberculosis clinical strain. Journal of Global Antimicrobial Resistance. Vol.3, No.4 (2015), 262-266. doi:10.1016/j.jgar.2015.07.004 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/36045
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Title
Genetic characterisation of a whiB7 mutant of a Mycobacterium tuberculosis clinical strain
Abstract
© 2015 International Society for Chemotherapy of Infection and Cancer. Mycobacterium tuberculosis is naturally resistant to clarithromycin (CLR). The genes Rv3197A (whiB7) and Rv1988 (ermMT) have been shown to be involved in the resistant phenotype. In this study, a CLR-susceptible M. tuberculosis clinical strain was identified, designated as DS3214, and the nucleotide sequences and expression profiles of whiB7 and ermMT were investigated. The results revealed that strain DS3214 contained a one nucleotide deletion in whiB7, leading to a truncated peptide. Expression of whiB7 was low, whereas comparable expression of ermMT was determined compared with the reference strain M. tuberculosis H37Rv. Overexpression of the mutant whiB7 in M. tuberculosis H37Ra did not increase the minimum inhibitory concentration (MIC) to CLR or kanamycin, indicating the defect of the mutant WhiB7. The CLR-susceptible M. tuberculosis clinical strain, whose whiB7 is naturally mutated, was first described in this study and whiB7 has been shown to play a role in the CLR-susceptible phenotype.