Publication:
Evaluation of a portable microchip electrophoresis fluorescence detection system for the analysis of amino acid neurotransmitters in brain dialysis samples

Issued Date

2016-01-01

Resource Type

Article

ISSN

13482246
09106340

DOI

10.2116/analsci.32.35

Other identifier(s)

2-s2.0-84959272139

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Analytical Sciences. Vol.32, No.1 (2016), 35-40

Suggested Citation

Nathan J. Oborny, Elton E.Melo Costa, Leena Suntornsuk, Fabiane C. Abreu, Susan M. Lunte Evaluation of a portable microchip electrophoresis fluorescence detection system for the analysis of amino acid neurotransmitters in brain dialysis samples. Analytical Sciences. Vol.32, No.1 (2016), 35-40. doi:10.2116/analsci.32.35 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43427

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Title

Evaluation of a portable microchip electrophoresis fluorescence detection system for the analysis of amino acid neurotransmitters in brain dialysis samples

Author(s)

Nathan J. Oborny
 Elton E.Melo Costa
 Leena Suntornsuk
 Fabiane C. Abreu
 Susan M. Lunte

Other Contributor(s)

University of Kansas
 Universidade Federal de Alagoas
 Mahidol University

Abstract

© 2016 The Japan Society for Analytical Chemistry. A portable fluorescence detection system for use with microchip electrophoresis was developed and compared to a benchtop system. Using this system, six neuroactive amines commonly found in brain dialysate (arginine, citrulline, taurine, histamine, glutamate, and aspartate) were derivatized offline with naphthalene-2,3-dicarboxaldehyde/cyanide, separated electrophoretically, and detected by fluorescence. The limits of detection for the analytes of interest were 50 - 250 nM for the benchtop system and 250 nM - 1.3 μM for the portable system, both of which were adequate for most analyte detection in brain microdialysis samples. The portable system was then demonstrated for the detection of the same six amines in a rat brain microdialysis sample.

Keyword(s)

Chemistry

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/43427

Collections

Scopus 2016-2017