Publication: Expression of Penaeus monodon ortholog of Niemann-Pick type C-2 in the spermatic tract, and its role in sperm cholesterol removal
Issued Date
2016-03-01
Resource Type
ISSN
10982795
1040452X
1040452X
Other identifier(s)
2-s2.0-84958718938
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular Reproduction and Development. Vol.83, No.3 (2016), 259-270
Suggested Citation
Charoonroj Chotwiwatthanakun, Jutharat Sangatit, Wanida Santimanawong, Piyaporn Surinlert, Juthatip Prommoon, Wattana Weerachatyanukul, Boonsirm Withyachumnarnkul, Rapeepun Vanichviriyakit Expression of Penaeus monodon ortholog of Niemann-Pick type C-2 in the spermatic tract, and its role in sperm cholesterol removal. Molecular Reproduction and Development. Vol.83, No.3 (2016), 259-270. doi:10.1002/mrd.22621 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43073
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Title
Expression of Penaeus monodon ortholog of Niemann-Pick type C-2 in the spermatic tract, and its role in sperm cholesterol removal
Abstract
© 2016 Wiley Periodicals, Inc. Protein and lipid composition of sperm plasma membrane are modified as these gametes continue to mature during their transit along the spermatic tract. Our previous study revealed that during its journey through the spermatic duct of the black tiger prawn, Penaeus monodon, sperm cholesterol content decreases through the action of lipid-binding proteins within the luminal environment. In this study, the full cDNA sequence of epididymal secretory protein E1 (HE1), or Niemann-Pick C2 (NPC2), was cloned from P. monodon (termed Pmnpc2), and its conserved cholesterol/lipid-binding domain was characterized. The putative tertiary structure of PmNPC2 showed high similarity with the structure of Bos taurus NPC2. Pmnpc2 is expressed in many tissues, including the spermatic tract (i.e., testis, vas deferens, terminal ampoule) and the female thelycum. In situ hybridization revealed the presence of Pmnpc2 transcripts in the vas deferens, terminal ampoule, and thelycum epithelia, suggesting that PmNPC2 could be secreted into the lumen of the spermatic duct. A recombinant hexahistidine-tagged PmNPC2 (rPmNPC2-6His) was able to bind cholesterol and sperm lipid extracts, while co-incubation of sperm from the vas deferens with rPmNPC2-6His resulted in the depletion of cholesterol from these gametes. Together, these results suggest that PmNPC2 participates in sperm cholesterol efflux during the sperm maturation process in P. monodon.