Publication: Structural and promoter regions of the murine pyruvate carboxylase gene
Issued Date
2001-09-21
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ISSN
0006291X
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2-s2.0-0035929365
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.287, No.2 (2001), 411-417
Suggested Citation
Sarawut Jitrapakdee, Nathida Petchamphai, Piyanate Sunyakumthorn, John C. Wallace, Vichai Boonsaeng Structural and promoter regions of the murine pyruvate carboxylase gene. Biochemical and Biophysical Research Communications. Vol.287, No.2 (2001), 411-417. doi:10.1006/bbrc.2001.5599 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/26444
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Title
Structural and promoter regions of the murine pyruvate carboxylase gene
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Abstract
We have cloned and sequenced the gene encoding mouse pyruvate carboxylase (mPC) [EC 6.4.1.1]. The coding region contains 19 exons, one 5′-untranslated region exon, and 19 introns in 22 kb of genomic DNA. This gene's exon/intron organization is highly conserved with respect to rat and human PC genes. The mPC gene promoter lacks canonical TATA and CCAAT boxes, in common with a number of housekeeping genes. Transient expressions in COS-1 of a luciferase reporter gene under the control of 5′-nested deletions of the 5′-flanking sequence of the mPC gene have identified the 166-bp minimal sequence required for basal transcription. Alternative splicing at the 5′-untranslated region exon of the mouse PC gene results in the production of two alternate transcripts bearing different 5′-noncoding regions. Both transcripts are highly expressed in kidney and liver and moderately expressed in heart and testis and expressed at a low level in spleen. © 2001 Academic Press.