Publication: Evaluation of a pharmacogenetic test in thailand for abacavir hypersensitivity screening in human immunodeficiency virus infection
18
Issued Date
2013-01-01
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ISSN
18756913
18756921
18756921
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2-s2.0-84888086737
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Mahidol University
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SCOPUS
Bibliographic Citation
Current Pharmacogenomics and Personalized Medicine. Vol.11, No.3 (2013), 231-236
Suggested Citation
Chonlaphat Sukasem, Montchai Gatrungsei, Somying Promso, Phillip Cunningham, Napatrupron Koomdee, Siwalee Santon, Montri Chamnanphon, Insee Sensorn, Wasun Chantratita Evaluation of a pharmacogenetic test in thailand for abacavir hypersensitivity screening in human immunodeficiency virus infection. Current Pharmacogenomics and Personalized Medicine. Vol.11, No.3 (2013), 231-236. doi:10.2174/18756921113119990005 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31420
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Title
Evaluation of a pharmacogenetic test in thailand for abacavir hypersensitivity screening in human immunodeficiency virus infection
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Abstract
Abacavir hypersensitivity reaction (ABC-HSR) is associated with the presence of HLA-B* 57:01 allele. Alternative tests for ABC-HSR associated allele determination are needed where sequence-based HLA typing is not available, particularly in resource-limited settings or developing countries. This study focused on the development and evaluation of two HLA-B* 57:01 tagging SNPs (single nucleotide polymorphism) HCP5 (HLA complex P5) rs2395029 and TNF (tumor necrosis factor) rs3093726 genotyping assays. Two hundred and thirteen purified genomic DNA samples were used to evaluate the performance characteristics of a HLA-B* 57:01 screening method based on allele-specific polymerase chain reaction (AS-PCR) with melting curve analysis. HCP5 rs2395029 and TNF rs3093726 were also genotyped using simple probe real-time PCR assay. All samples were successfully genotyped wherein AS-PCR genotyping provided 100% sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) when compared with specific HLA-B status by sequencing based assay. When comparing the AS-PCR screening method with the HCP5 rs2395029 and TNF rs3093726 genotyping method, the former had 100% sensitivity, 100% specificity, 100% PPV and 100% NPV using a simple probe; while the latter one had 95.24% sensitivity, 100% specificity, 100% PPV and 99.48% NPV, respectively. In conclusion, our study lends support on a molecular tool for pharmacogenetic screening in resource-limited settings. Thus, serious drug hypersensitivity associated with ABC may potentially be reduced in Thailand by further evaluation of the proposed assay in clinical practice. © 2013 Bentham Science Publishers.