Publication: Molecular Epidemiology of Hemoglobinopathies in Cambodia
Issued Date
2016-05-03
Resource Type
ISSN
1532432X
03630269
03630269
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2-s2.0-84971628902
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Mahidol University
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SCOPUS
Bibliographic Citation
Hemoglobin. Vol.40, No.3 (2016), 163-167
Suggested Citation
Thongperm Munkongdee, Jatuporn Tanakulmas, Punnee Butthep, Pranee Winichagoon, Barbara Main, Miriam Yiannakis, Joby George, Robyn Devenish, Suthat Fucharoen, Saovaros Svasti Molecular Epidemiology of Hemoglobinopathies in Cambodia. Hemoglobin. Vol.40, No.3 (2016), 163-167. doi:10.3109/03630269.2016.1158723 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/43048
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Title
Molecular Epidemiology of Hemoglobinopathies in Cambodia
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Abstract
© 2016 Informa UK Limited, trading as Taylor & Francis Group. Determining the magnitude of the thalassemia problem in a country is important for implementing a national prevention and control program. In order to acquire accurate thalassemia prevalence data, the gene frequency of α- and β-thalassemia (α- and β-thal) in different regions of a country should be determined. The molecular basis of thalassemia in Cambodia was performed by polymerase chain reaction (PCR)-based techniques in a community-based cross-sectional survey of 1631 unrelated individuals from three regions, Battambang, Preah Vihear and Phnom Penh. Thalassemia mutations were detected in 62.7% of the three studied population of Cambodia. Hb E (HBB: c.79G > A) was the most common β-globin gene mutation with a frequency ranging from 0.139 to 0.331, while the most frequent α-globin gene mutation was the -α3.7(rightward) deletion (0.098-0.255). The other frequencies were 0.001-0.003 for β-thal, 0.008-0.011 for α-thal-1 (- -SEA), 0.003-0.008 for α-thal-2 [-α4.2(leftward deletion)], 0.021-0.044 for Hb Constant Spring (Hb CS, HBA2: c.427T > C) and 0.009-0.036 for Hb Paksé (HBA2: c.429A > T). A regional specific thalassemia gene frequency was observed. Preah Vihear had the highest prevalence of Hb E (55.9%), α-thal-2 (24.0%) and nondeletional α-thal (15.1%), whereas Phnom Penh had the lowest frequency of thalassemia genes. Interestingly, in Preah Vihear, the frequency of Hb Paksé was extremely high (0.036), almost equivalent to that of Hb CS (0.044). Our results indicate the importance of micromapping and epidemiology studies of thalassemia, which will assist in establishing the national prevention and control program in Cambodia.