Publication:
Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire

Issued Date

2010-07-13

Resource Type

Article

ISSN

1474760X
14747596

DOI

10.1186/gb-2010-11-7-r73

Other identifier(s)

2-s2.0-77954417202

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Genome Biology. Vol.11, No.7 (2010)

Suggested Citation

C. André Lévesque, Henk Brouwer, Liliana Cano, John P. Hamilton, Carson Holt, Edgar Huitema, Sylvain Raffaele, Gregg P. Robideau, Marco Thines, Joe Win, Marcelo M. Zerillo, Gordon W. Beakes, Jeffrey L. Boore, Dana Busam, Bernard Dumas, Steve Ferriera, Susan I. Fuerstenberg, Claire M.M. Gachon, Elodie Gaulin, Francine Govers, Laura Grenville-Briggs, Neil Horner, Jessica Hostetler, Rays H.Y. Jiang, Justin Johnson, Theerapong Krajaejun, Haining Lin, Harold J.G. Meijer, Barry Moore, Paul Morris, Vipaporn Phuntmart, Daniela Puiu, Jyoti Shetty, Jason E. Stajich, Sucheta Tripathy, Stephan Wawra, Pieter van West, Brett R. Whitty, Pedro M. Coutinho, Bernard Henrissat, Frank Martin, Paul D. Thomas, Brett M. Tyler, Ronald P. De Vries, Sophien Kamoun, Mark Yandell, Ned Tisserat, C. Robin Buell Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire. Genome Biology. Vol.11, No.7 (2010). doi:10.1186/gb-2010-11-7-r73 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28482

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Title

Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire

Author(s)

C. André Lévesque
 Henk Brouwer
 Liliana Cano
 John P. Hamilton
 Carson Holt
 Edgar Huitema
 Sylvain Raffaele
 Gregg P. Robideau
 Marco Thines
 Joe Win
 Marcelo M. Zerillo
 Gordon W. Beakes
 Jeffrey L. Boore
 Dana Busam
 Bernard Dumas
 Steve Ferriera
 Susan I. Fuerstenberg
 Claire M.M. Gachon
 Elodie Gaulin
 Francine Govers
 Laura Grenville-Briggs
 Neil Horner
 Jessica Hostetler
 Rays H.Y. Jiang
 Justin Johnson
 Theerapong Krajaejun
 Haining Lin
 Harold J.G. Meijer
 Barry Moore
 Paul Morris
 Vipaporn Phuntmart
 Daniela Puiu
 Jyoti Shetty
 Jason E. Stajich
 Sucheta Tripathy
 Stephan Wawra
 Pieter van West
 Brett R. Whitty
 Pedro M. Coutinho
 Bernard Henrissat
 Frank Martin
 Paul D. Thomas
 Brett M. Tyler
 Ronald P. De Vries
 Sophien Kamoun
 Mark Yandell
 Ned Tisserat
 C. Robin Buell

Other Contributor(s)

Agriculture et Agroalimentaire Canada
 Carleton University
 CBS Fungal Biodiversity Center CBS - KNAW
 The Sainsbury Laboratory
 Michigan State University
 University of Utah Health
 Biodiversity and Climate Research Centre
 Goethe-Universitat Frankfurt am Main
 Colorado State University
 Newcastle University, United Kingdom
 Genome Project Solutions
 J. Craig Venter Institute
 Universite de Toulouse
 The Scottish Association for Marine Science
 Wageningen University and Research Centre
 Centre for Biosystems Genomics, Wageningen
 University of Aberdeen School of Medicine
 Broad Institute
 Mahidol University
 Bowling Green State University
 University of California, Riverside
 Biocomplexity Institute of Virginia Tech
 Universite de Provence Aix-Marseille 1
 USDA Agricultural Research Service, Washington DC
 SRI International

Abstract

Background: Pythium ultimum is a ubiquitous oomycete plant pathogen responsible for a variety of diseases on a broad range of crop and ornamental species.Results: The P. ultimum genome (42.8 Mb) encodes 15,290 genes and has extensive sequence similarity and synteny with related Phytophthora species, including the potato blight pathogen Phytophthora infestans. Whole transcriptome sequencing revealed expression of 86% of genes, with detectable differential expression of suites of genes under abiotic stress and in the presence of a host. The predicted proteome includes a large repertoire of proteins involved in plant pathogen interactions, although, surprisingly, the P. ultimum genome does not encode any classical RXLR effectors and relatively few Crinkler genes in comparison to related phytopathogenic oomycetes. A lower number of enzymes involved in carbohydrate metabolism were present compared to Phytophthora species, with the notable absence of cutinases, suggesting a significant difference in virulence mechanisms between P. ultimum and more host-specific oomycete species. Although we observed a high degree of orthology with Phytophthora genomes, there were novel features of the P. ultimum proteome, including an expansion of genes involved in proteolysis and genes unique to Pythium. We identified a small gene family of cadherins, proteins involved in cell adhesion, the first report of these in a genome outside the metazoans.Conclusions: Access to the P. ultimum genome has revealed not only core pathogenic mechanisms within the oomycetes but also lineage-specific genes associated with the alternative virulence and lifestyles found within the pythiaceous lineages compared to the Peronosporaceae. © 2010 Lévesque et al.; licensee BioMed Central Ltd.

Keyword(s)

Agricultural and Biological Sciences
 Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/28482

Collections

Scopus 2006-2010