Publication: Dehydration effects on cryopreservation of Cymbidium finlaysonianum protocorms
2
Issued Date
2019-01-01
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ISSN
24066168
05677572
05677572
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2-s2.0-85064073548
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Mahidol University
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SCOPUS
Bibliographic Citation
Acta Horticulturae. Vol.1234, (2019), 133-136
Suggested Citation
S. Samala, K. Thammasiri Dehydration effects on cryopreservation of Cymbidium finlaysonianum protocorms. Acta Horticulturae. Vol.1234, (2019), 133-136. doi:10.17660/ActaHortic.2019.1234.17 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/49857
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Title
Dehydration effects on cryopreservation of Cymbidium finlaysonianum protocorms
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Abstract
© 2019 International Society for Horticultural Science. All Rights Reserved. Protocorms of Cymbidium finlaysonianum cultured on MS agar medium without plant growth regulator were dehydrated on cryo-plates with PVS2, PVS3 and glycerol for 0, 30, 60, 90 and 120 min prior to being plunged into liquid nitrogen (LN) for 1 h. The results showed that treated controls (PVS2, PVS3 and glycerol without LN) gave lower survival when the exposure times were longer. The PVS2 and PVS3 exposure time for 60 min prior to plunging into LN gave the highest survival of 40 and 10%, respectively. There is no survival after cryopreservation when glycerol was used for dehydration. After that, cryopreserved protocorms developed into plantlets within 3 months of culture.