Publication: Evaluation of a monoclonal antibody-based dot-blot ELISA for detection of leptospira spp inbovine urine samples
Issued Date
2005-05-01
Resource Type
ISSN
00029645
Other identifier(s)
2-s2.0-20444463125
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Veterinary Research. Vol.66, No.5 (2005), 762-766
Suggested Citation
Junpen Suwimonteerabutr, Wanpen Chaicumpa, Patcharin Saengjaruk, Pramuan Tapchaisri, Manas Chongsa-nguan, Thareerat Kalambaheti, Pongrama Ramasoota, Yuwaporn Sakolvaree, Prachin Virakul Evaluation of a monoclonal antibody-based dot-blot ELISA for detection of leptospira spp inbovine urine samples. American Journal of Veterinary Research. Vol.66, No.5 (2005), 762-766. doi:10.2460/ajvr.2005.66.762 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/17199
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Evaluation of a monoclonal antibody-based dot-blot ELISA for detection of leptospira spp inbovine urine samples
Other Contributor(s)
Abstract
Objective - To evaluate the efficacy of a novel monoclonal antibody (MAb)-based dot-blot ELISA for detection of Leptospira antigens in urine samples of cattle. Sample population - Blood and urine samples of 45 test cattle from 5 farms in Chonburi province and 20 control cattle from 2 farms in Khon Kaen province in Thailand. Procedure - Blood and urine samples were assayed (microscopic agglutination test and urine antigen test) for Leptospira infection by use of an MAb-based dot-blot ELISA, and results for the ELISA were compared with those for dark-field microscopy (DFM), microbial culture, and a polymerase chain reaction (PCR) assay. Results - All urine samples with positive results for DFM, microbial culture, PCR assay, or > 1 of these tests also had positive results when tested by use of the MAb-based dot-blot ELISA, except for 1 sample that had positive results only for the PCR assay. Detection limits of the dot-blot ELISA were 103 leptospires/mL of urine and 9.3 ng of Leptospira homogenate. Comparison revealed that the diagnostic sensitivity, specificity, efficacy (accuracy), positive predictive value, and negative predictive value for the ELISA were in agreement with results for DFM (100%, 72.72%, 80%, 57.14%, and 100%, respectively), microbial culture (100%, 61.54%, 66.62%, 28.57%, and 100%, respectively), and PCR assay (95.45%, 100%, 91.77%, 100%, and 95.83%, respectively). Conclusions and clinical relevance - The MAb-based dot-blot ELISA is suitable as a tool for detecting leptospires in urine samples of cattle.