Publication: A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis
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Issued Date
2016-10-21
Resource Type
ISSN
13645528
00032654
00032654
Other identifier(s)
2-s2.0-84990061917
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Mahidol University
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SCOPUS
Bibliographic Citation
Analyst. Vol.141, No.20 (2016), 5767-5775
Suggested Citation
Pawasuth Saengdee, Woraphan Chaisriratanakul, Win Bunjongpru, Witsaroot Sripumkhai, Awirut Srisuwan, Charndet Hruanun, Amporn Poyai, Ponrut Phunpae, Supansa Pata, Wutthinan Jeamsaksiri, Watchara Kasinreak, Chamras Promptmas A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis. Analyst. Vol.141, No.20 (2016), 5767-5775. doi:10.1039/c6an00568c Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/42962
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Title
A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis
Abstract
© 2016 The Royal Society of Chemistry. A silicon nitride Ion Sensitive Field Effect Transistor (ISFET) based immunosensor was developed as a low-cost and label-free electrical detection for the detection of antigen 85 complex B (Ag85B). The sensing membrane of the ISFET was modified with 3-aminopropyltriethoxysilane (APTES) followed by glutaraldehyde (GA), yielding an aldehyde-terminated surface. This group is available for immobilization of a monoclonal antibody against a recombinant Ag85B protein (anti-Ag85B antibody). The optimal concentration for anti-Ag85B antibody immobilization onto the modified ISFET was 100 μg ml-1. This optimal condition provided the maximal binding capability and minimal non-specific background signal. The binding event between the recombinant Ag85B antigen and anti-Ag85B antibody on the ISFET surface is presented by monitoring the gate potential change at a constant drain current. The dose response for the recombinant Ag85B protein showed a linear response between 0.12 and 1 μg ml-1without significant interference from other recombinant proteins. The analytical imprecision (CV%) and accuracy of this Ag85B protein biosensor were 9.73-10.99% and 95.29%, respectively. In addition, an irrelevant antibody and other recombinant proteins were employed as a negative control to demonstrate the non-specific interaction of the antigen and antibody. The success of this immunosensor system for Ag85B protein detection facilitates the construction of a promising device which can shorten the turnaround time for the diagnosis of tuberculosis compared to a standard culture method. Furthermore, this device could also be applied for real-time growth monitoring of Mycobacterium tuberculosis in a mycobacterial culture system.