Polyclonal antibodies were produced by immunization of rabbit with inactivated avian influenza
subtype H5N1, isolated in Thailand in 2004, combined with Freund's or Alum adjuvant. Polyclonal antibodies
from rabbit inhibited hemagglutination activity of H5N1 virus. Rabbit immunized by using 10% formalin
inactivated virus emulsify with Freund's adjuvant showed strong immune response to the virus and the
highest antibody titer was 1:320.