Publication: Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum-free embryoid bodies
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Issued Date
2015-01-01
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ISSN
15221563
03636143
03636143
Other identifier(s)
2-s2.0-84922262889
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Physiology - Cell Physiology. Vol.308, No.3 (2015), C209-C219
Suggested Citation
Michael W. Nestor, Samson Jacob, Bruce Sun, Deborah Prè, Andrew A. Sproul, Seong Im Hong, Chris Woodard, Matthew Zimmer, Vorapin Chinchalongporn, Ottavio Arancio, Scott A. Noggle Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum-free embryoid bodies. American Journal of Physiology - Cell Physiology. Vol.308, No.3 (2015), C209-C219. doi:10.1152/ajpcell.00263.2014 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/35626
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Title
Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum-free embryoid bodies
Abstract
© 2015 the American Physiological Society. Production and isolation of forebrain interneuron progenitors are essential for understanding cortical development and developing cell-based therapies for developmental and neurodegenerative disorders. We demonstrate production of a population of putative calretinin-positive bipolar interneurons that express markers consistent with caudal ganglionic eminence identities. Using serum-free embryoid bodies (SFEBs) generated from human inducible pluripotent stem cells (iPSCs), we demonstrate that these interneuron progenitors exhibit morphological, immunocytochemical, and electrophysiological hallmarks of developing cortical interneurons. Finally, we develop a fluorescence-activated cell-sorting strategy to isolate interneuron progenitors from SFEBs to allow development of a purified population of these cells. Identification of this critical neuronal cell type within iPSC-derived SFEBs is an important and novel step in describing cortical development in this iPSC preparation.