Publication: Monoclonal antibody against Fasciola gigantica glutathione peroxidase and their immunodiagnosis potential for fasciolosis
Issued Date
2019-12-01
Resource Type
ISSN
18732550
03044017
03044017
Other identifier(s)
2-s2.0-85075377658
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Veterinary Parasitology. Vol.276, (2019)
Suggested Citation
Pornanan Kueakhai, Kulathida Chaithirayanon, Athit Chaiwichien, Tepparit Samrit, Supawadee Osotprasit, Phawiya Suksomboon, Wipaphorn Jaikua, Prasert Sobhon, Narin Changklungmoa Monoclonal antibody against Fasciola gigantica glutathione peroxidase and their immunodiagnosis potential for fasciolosis. Veterinary Parasitology. Vol.276, (2019). doi:10.1016/j.vetpar.2019.108979 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50982
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Monoclonal antibody against Fasciola gigantica glutathione peroxidase and their immunodiagnosis potential for fasciolosis
Other Contributor(s)
Abstract
© 2019 Elsevier B.V. Glutathione peroxidases (GPx), major antioxidant enzymes, secreted by Fasciola spp., are important for the parasite evasion and protection against the host's immune responses. In the present study, a monoclonal antibody (MoAb) against recombinant F. gigantica glutathione peroxidase (rFgGPx) was produced by hybridoma technique using spleen cells from BALB/c mice immunized with rFgGPx. This MoAb (named 7B8) is IgG1 with κ light chains, and it reacted specifically with rFgGPx at a molecular weight 19 kDa as shown by immunoblotting, and reacted with the native FgGPx in the extracts of whole body (WB), metacercariae, newly excysted juveniles (NEJs), 4 week-old juveniles and adult F. gigantica as shown by indirect ELISA. It did not cross react with antigens in WB fractions from other adult trematodes, including Fischoederius cobboldi, Paramphistomum cervi, Setaria labiato-papillosa, Eurytrema pancreaticum, Gastrothylax crumenifer and Gigantocotyle explanatum. By immunolocalization, MoAb against rFgGPx reacted with the native protein in the tegument, vitelline cells, and eggs of adult F. gigantica. In addition, the sera from mice experimentally infected with F. gigantica were tested positive by this indirect sandwich ELISA. This result indicated that FgGPx is an abundantly expressed parasite protein that is secreted into the tegumental antigens (TA), therefore, FgGPx and its MoAb may be used for immunodiagnosis of both early and late fasciolosis gigantica in animals and humans.