Application of AFLP technique to the study of calving interval trait of thai swamp buffalo (Bubalus bubalis)

Abstract

This study aimed to develop genetic markers specific for the calving interval (CI) trait of the domestic Thai buffalo (Bubalus bubalis) using the technique of Amplified Fragment Length Polymorphism (AFLP). Using two groups of buffalo with extreme CI phenotypes, several AFLP markers were identified. The DNA sequences of all identified polymorphic AFLP fragments were determined by DNA sequencing and analyzed with BLAST searches. Interestingly, the analysis showed that the sequence of the AFE7M24-2 marker has a high similarity with the prostaglandin f2α receptor gene, while the sequences of the AFE16M25-2 and AFE4M28-3 markers have high similarity with the follicle stimulating hormone receptor gene of sheep (Ovis aries).