Publication: Glutathione transferase Omega 1-1 (GSTO1-1) modulates Akt and MEK1/2 signaling in human neuroblastoma cell SH-SY5Y
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Issued Date
2019-07-01
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ISSN
10970134
08873585
08873585
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2-s2.0-85063344312
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Mahidol University
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SCOPUS
Bibliographic Citation
Proteins: Structure, Function and Bioinformatics. Vol.87, No.7 (2019), 588-595
Suggested Citation
Chonticha Saisawang, Jantana Wongsantichon, Robert C. Robinson, Albert J. Ketterman Glutathione transferase Omega 1-1 (GSTO1-1) modulates Akt and MEK1/2 signaling in human neuroblastoma cell SH-SY5Y. Proteins: Structure, Function and Bioinformatics. Vol.87, No.7 (2019), 588-595. doi:10.1002/prot.25683 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/50141
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Title
Glutathione transferase Omega 1-1 (GSTO1-1) modulates Akt and MEK1/2 signaling in human neuroblastoma cell SH-SY5Y
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Abstract
© 2019 Wiley Periodicals, Inc. In the human neuroblastoma SH-SY5Y cell line, the glutathione transferase Omega 1-1 (GSTO1-1) appears to modulate Akt and MEK1/2 kinase activation. We observed a glutathionylation modification was involved in the activation of Akt but not MEK1/2. With the specific GSTO1-1 inhibitor ML175, we show the enzyme activity of GSTO1-1 is important for modulation as the inhibited GSTO1-1 allowed activation of both Akt and MEK1/2. The inhibition of GSTO1-1 showed a similar extent of activation of Akt and MEK1/2 as treatment by the endotoxin lipopolysaccharide. The GSTO1-1 also either directly interacts with Akt and MEK1/2 or interacts with a protein complexed with Akt and MEK1/2 as both kinases coimmunoprecipitated with GSTO1-1. The results suggest that GSTO1-1 enzyme activity inhibits the activation of these two kinases to maintain basal levels. The possible regulation by GSTO1-1 is of interest as both kinases have hundreds of potential downstream targets that are known to have contributions to various cellular processes including survival, growth, proliferation, and metabolism.