Publication: Specific monoclonal antibodies to Opisthorchis viverrini
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Issued Date
1991-01-01
Resource Type
ISSN
00207519
Other identifier(s)
2-s2.0-0026323124
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal for Parasitology. Vol.21, No.8 (1991), 969-974
Suggested Citation
Wanpen Chaicumpa, Yuwaporn Ruangkunaporn, Thareerat Kalambaheti, Suvit Limavongpranee, Viroj Kitikoon, Srisin Khusmith, Swangjai Pungpak, Manas Chongsa-Nguan, Santasiri Sornmani Specific monoclonal antibodies to Opisthorchis viverrini. International Journal for Parasitology. Vol.21, No.8 (1991), 969-974. doi:10.1016/0020-7519(91)90175-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/22055
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Title
Specific monoclonal antibodies to Opisthorchis viverrini
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Abstract
A Balb/c mouse was immunized with a crude soluble antigen of Opisthorchis viverrini adult worms (OVAA) over a period of 7 months. Spleen cells from the immune mouse were fused with Sp2/0 myeloma cells. Among the 264 tissue culture wells containing the fused cells, cells of 96 wells (36%) produced antibodies to the immunizing agent. Antibodies produced by cells in several wells reacted with antigens from other species of parasite. Cells of 17 wells produced antibodies specific only to OVAA, thus cells from three representative wells were cloned by limiting dilution. Hybrids obtained produced antibodies which could be classified according to their tissue specificities into three groups. The first group of antibodies reacted strongly to the worm integument and weakly with the muscles while those belonging to the second group reacted only to muscles of the worms. The monoclonal antibodies of the third group gave a positive reaction to both muscles and tegument. © 1991.