Publication: Chronic kidney disease impairs bone defect healing in rats.
Accepted Date
2016-02-29
Issued Date
2016-03-09
Resource Type
Language
eng
ISSN
2045-2322 (electronic)
Rights
Mahidol University
Rights Holder(s)
Nature Publishing Group
Bibliographic Citation
Liu W, Kang N, Seriwatanachai D, Dong Y, Zhou L, Lin Y, et al. Chronic kidney disease impairs bone defect healing in rats. Scientific Reports. 2016 Mar 9;6:23041.
Suggested Citation
Dutmanee Seriwatanachai, ดุษมณี เสรีวัฒนาชัย, Liu, Weiqing, Kang, Ning, Dong, Yuliang, Zhou, Liyan, Lin, Yunfeng, Ye, Ling, Liang, Xing, Yuan, Quan Chronic kidney disease impairs bone defect healing in rats.. Liu W, Kang N, Seriwatanachai D, Dong Y, Zhou L, Lin Y, et al. Chronic kidney disease impairs bone defect healing in rats. Scientific Reports. 2016 Mar 9;6:23041.. doi:10.1038/srep23041 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/1022
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Title
Chronic kidney disease impairs bone defect healing in rats.
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Abstract
Chronic kidney disease (CKD) has been regarded as a risk for bone health. The aim of this study was to evaluate the effect of CKD on bone defect repair in rats. Uremia was induced by subtotal renal ablation, and serum levels of BUN and PTH were significantly elevated four weeks after the second renal surgery. Calvarial defects of 5-mm diameter were created and implanted with or without deproteinized bovine bone mineral (DBBM). Micro-CT and histological analyses consistently revealed a decreased newly regenerated bone volume for CKD rats after 4 and 8 weeks. In addition, 1.4-mm-diameter cortical bone defects were established in the distal end of femora and filled with gelatin sponge. CKD rats exhibited significantly lower values of regenerated bone and bone mineral density (BMD) within the cortical gap after 2 and 4 weeks. Moreover, histomorphometric analysis showed an increase in both osteoblast number (N.Ob/B.Pm) and osteoclast number (N.Oc/B.Pm) in CKD groups due to hyperparathyroidism. Notably, collagen maturation was delayed in CKD rats as verified by Masson’s Trichrome staining. These data indicate that declined renal function negatively affects bone regeneration in both calvarial and femoral defects.