Publication: Production of carbazole alkaloids through callus and suspension cultures in Clausena harmandiana
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Issued Date
2018-01-01
ISSN
14786427
14786419
14786419
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2-s2.0-85059052930
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Mahidol University
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SCOPUS
Bibliographic Citation
Natural Product Research. (2018)
Suggested Citation
Tharita Kitisripanya, Manus Laoburee, Luksami Puengsiricharoen, Pukpicha Pratoomtong, Supawadee Daodee, Jinda Wangboonskul, Waraporn Putalun Production of carbazole alkaloids through callus and suspension cultures in Clausena harmandiana. Natural Product Research. (2018). doi:10.1080/14786419.2018.1533833 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/44885
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Title
Production of carbazole alkaloids through callus and suspension cultures in Clausena harmandiana
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Abstract
© 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group. Carbazole alkaloids are major constituents in Clausena spp. and exhibit a wide range of biological activities. The roots of Clausena harmandiana are a rich source of active carbazole alkaloids. However, its roots take several years to grow to be able to harvest. To obtain an alternative source of carbazole alkaloids, in vitro callus cultures of C. harmandiana were induced, and the formation of two active carbazole alkaloids was investigated. The effects of precursor, concentrations of sucrose, elicitors and light were studied to improve carbazole alkaloids formation. In this study, light had a strong effect on the formation of both carbazole alkaloids. The highest yields of clausine K and 7-methoxymukonal were 4.74 ± 0.26 and 0.92 ± 0.04 mg/g DW, respectively, which have more than 10-fold found in intact roots. According to the results of this study, C. harmandiana callus cultures can be used as an alternative source of carbazole alkaloids for additional biological studies.